Cooperative interplay of let-7 mimic and HuR with MYC RNA

Menachem J Gunzburg, Andrew Sivakumaran, Nicole R Pendini, Je-Hyun Yoon, Myriam Gorospe, Matthew C J Wilce, Jacqueline A Wilce

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15 Citations (Scopus)

Abstract

Both RNA-binding proteins (RBP) and miRNA play important roles in the regulation of mRNA expression, often acting together to regulate a target mRNA. In some cases the RBP and miRNA have been reported to act competitively, but in other instances they function cooperatively. Here, we investigated HuR function as an enhancer of let-7-mediated translational repression of c-Myc despite the separation of their binding sites. Using an in vitro system, we determined that a let-7 mimic, consisting of single-stranded (ss)DNA complementary to the let-7 binding site, enhanced the affinity of HuR for a 122-nt MYC RNA encompassing both binding sites. This finding supports the biophysical principle of cooperative binding by an RBP and miRNA purely through interactions at distal mRNA binding sites.
Original languageEnglish
Pages (from-to)2729 - 2733
Number of pages5
JournalCell Cycle
Volume14
Issue number17
DOIs
Publication statusPublished - 2015

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