Conventional PKCs regulate the temporal pattern of Ca2+ oscillations at fertilization in mouse eggs

Guillaume Halet, Richard Tunwell, Scott J. Parkinson, John Carroll

Research output: Contribution to journalArticleResearchpeer-review

70 Citations (Scopus)

Abstract

In mammalian eggs, sperm-induced Ca2+ oscillations at fertilization are the primary trigger for egg activation and initiation of embryonic development. Identifying the downstream effectors that decode this unique Ca2+ signal is essential to understand how the transition from egg to embryo is coordinated. Here, we investigated whether conventional PKCs (cPKCs) can decode Ca2+ oscillations at fertilization. By monitoring the dynamics of GFP-labeled PKCα and PKCγ in living mouse eggs, we demonstrate that cPKCs translocate to the egg membrane at fertilization following a pattern that is shaped by the amplitude, duration, and frequency of the Ca2+ transients. In addition, we show that cPKC translocation is driven by the C2 domain when Ca2+ concentration reaches 1-3 μM. Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca 2+ oscillations, presumably via the regulation of store-operated Ca2+ entry.

Original languageEnglish
Pages (from-to)1033-1044
Number of pages12
JournalJournal of Cell Biology
Volume164
Issue number7
DOIs
Publication statusPublished - 29 Mar 2004
Externally publishedYes

Keywords

  • Calcium
  • GFP
  • Influx
  • Oscillations
  • PKC

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