Controlling integrin-based adhesion to a degradable electrospun fibre scaffold via SI-ATRP

Andrew E. Rodda, Francesca Ercole, Veronica Glattauer, David R. Nisbet, Kevin E. Healy, Andrew P. Dove, Laurence Meagher, John S. Forsythe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

While polycaprolactone (PCL) and similar polyesters are commonly used as degradable scaffold materials in tissue engineering and related applications, non-specific adsorption of environmental proteins typically precludes any control over the signalling pathways that are activated during cell adhesion to these materials. Here we describe the preparation of PCL-based fibres that facilitate cell adhesion through well-defined pathways while preventing adhesion via adsorbed proteins. Surface-initiated atom transfer radical polymerisation (SI-ATRP) was used to graft a protein-resistant polymer brush coating from the surface of fibres, which had been electrospun from a brominated PCL macroinitiator. This coating also provided alkyne functional groups for the attachment of specific signalling molecules via the copper-mediated azide-alkyne click reaction; in this case, a cyclic RGD peptide with high affinity for αvβ3 integrins. Mesenchymal stem cells were shown to attach to the fibres via the peptide, but did not attach in its absence, nor when blocked with soluble peptide, demonstrating the effective control of cell adhesion pathways.

Original languageEnglish
Pages (from-to)7314-7322
Number of pages9
JournalJournal of Materials Chemistry B
Volume4
Issue number45
DOIs
Publication statusPublished - 7 Dec 2016

Cite this

@article{c64a2fc173a14184adcfc870c4757fc0,
title = "Controlling integrin-based adhesion to a degradable electrospun fibre scaffold via SI-ATRP",
abstract = "While polycaprolactone (PCL) and similar polyesters are commonly used as degradable scaffold materials in tissue engineering and related applications, non-specific adsorption of environmental proteins typically precludes any control over the signalling pathways that are activated during cell adhesion to these materials. Here we describe the preparation of PCL-based fibres that facilitate cell adhesion through well-defined pathways while preventing adhesion via adsorbed proteins. Surface-initiated atom transfer radical polymerisation (SI-ATRP) was used to graft a protein-resistant polymer brush coating from the surface of fibres, which had been electrospun from a brominated PCL macroinitiator. This coating also provided alkyne functional groups for the attachment of specific signalling molecules via the copper-mediated azide-alkyne click reaction; in this case, a cyclic RGD peptide with high affinity for αvβ3 integrins. Mesenchymal stem cells were shown to attach to the fibres via the peptide, but did not attach in its absence, nor when blocked with soluble peptide, demonstrating the effective control of cell adhesion pathways.",
author = "Rodda, {Andrew E.} and Francesca Ercole and Veronica Glattauer and Nisbet, {David R.} and Healy, {Kevin E.} and Dove, {Andrew P.} and Laurence Meagher and Forsythe, {John S.}",
year = "2016",
month = "12",
day = "7",
doi = "10.1039/C6TB02444K",
language = "English",
volume = "4",
pages = "7314--7322",
journal = "Journal of Materials Chemistry B",
issn = "2050-750X",
publisher = "The Royal Society of Chemistry",
number = "45",

}

Controlling integrin-based adhesion to a degradable electrospun fibre scaffold via SI-ATRP. / Rodda, Andrew E.; Ercole, Francesca; Glattauer, Veronica; Nisbet, David R.; Healy, Kevin E.; Dove, Andrew P.; Meagher, Laurence; Forsythe, John S.

In: Journal of Materials Chemistry B, Vol. 4, No. 45, 07.12.2016, p. 7314-7322.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Controlling integrin-based adhesion to a degradable electrospun fibre scaffold via SI-ATRP

AU - Rodda, Andrew E.

AU - Ercole, Francesca

AU - Glattauer, Veronica

AU - Nisbet, David R.

AU - Healy, Kevin E.

AU - Dove, Andrew P.

AU - Meagher, Laurence

AU - Forsythe, John S.

PY - 2016/12/7

Y1 - 2016/12/7

N2 - While polycaprolactone (PCL) and similar polyesters are commonly used as degradable scaffold materials in tissue engineering and related applications, non-specific adsorption of environmental proteins typically precludes any control over the signalling pathways that are activated during cell adhesion to these materials. Here we describe the preparation of PCL-based fibres that facilitate cell adhesion through well-defined pathways while preventing adhesion via adsorbed proteins. Surface-initiated atom transfer radical polymerisation (SI-ATRP) was used to graft a protein-resistant polymer brush coating from the surface of fibres, which had been electrospun from a brominated PCL macroinitiator. This coating also provided alkyne functional groups for the attachment of specific signalling molecules via the copper-mediated azide-alkyne click reaction; in this case, a cyclic RGD peptide with high affinity for αvβ3 integrins. Mesenchymal stem cells were shown to attach to the fibres via the peptide, but did not attach in its absence, nor when blocked with soluble peptide, demonstrating the effective control of cell adhesion pathways.

AB - While polycaprolactone (PCL) and similar polyesters are commonly used as degradable scaffold materials in tissue engineering and related applications, non-specific adsorption of environmental proteins typically precludes any control over the signalling pathways that are activated during cell adhesion to these materials. Here we describe the preparation of PCL-based fibres that facilitate cell adhesion through well-defined pathways while preventing adhesion via adsorbed proteins. Surface-initiated atom transfer radical polymerisation (SI-ATRP) was used to graft a protein-resistant polymer brush coating from the surface of fibres, which had been electrospun from a brominated PCL macroinitiator. This coating also provided alkyne functional groups for the attachment of specific signalling molecules via the copper-mediated azide-alkyne click reaction; in this case, a cyclic RGD peptide with high affinity for αvβ3 integrins. Mesenchymal stem cells were shown to attach to the fibres via the peptide, but did not attach in its absence, nor when blocked with soluble peptide, demonstrating the effective control of cell adhesion pathways.

UR - http://www.scopus.com/inward/record.url?scp=84996761433&partnerID=8YFLogxK

U2 - 10.1039/C6TB02444K

DO - 10.1039/C6TB02444K

M3 - Article

VL - 4

SP - 7314

EP - 7322

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-750X

IS - 45

ER -