Controlling cell-material interactions using coatings with advanced polymer architectures

Peter Koegler, Paul J Pasic, Graham Johnson, Penny Bean, Guenter Lorenz, Laurence Meagher, Helmut Thissen

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

3 Citations (Scopus)

Abstract

Polymeric coatings which allow the effective control of biointerfacial interactions and cellular responses are of increasing interest in a range of biomedical applications in vitro and in vivo such as cell culture tools, biosensors and implantable medical devices. A variety of coating strategies have been developed to gain control over cell-surface interactions but many of them are limited with respect to their function and transferability between different substrate materials. Here, our aim was to establish an easily transferable coating that reduces non-specific cell-surface interactions to a minimum while at the same time presenting functional groups which allow for the subsequent immobilisation of bioactive signals. To achieve this, we have applied an allylamine plasma polymer coating followed by the covalent immobilisation of a macro-initiator providing iniferter functional groups. Subsequent controlled free radical graft polymerisation using the monomers acrylamide and acrylic acid in different molar ratios resulted in highly uniform polymer coatings. Non-specific cell attachment was significantly reduced on coatings representing molar ratios of less than 10 acrylic acid. At the same time we have demonstrated the suitability of these coatings for the subsequent covalent binding of bioactive compounds carrying amine functional groups using the label 2,2,2-trifluoroethylamine. Successful surface modifications were confirmed by X-ray photoelectron spectroscopy (XPS) and profilometry. The cellular response was evaluated using HeLa cell attachment experiments for up to 24 hours. We expect that the coating platform established in this study will be translated into a number of biomedical applications, including applications in implantable devices and regenerative medicine.
Original languageEnglish
Title of host publicationProceedings of SPIE: Smart Nano+Micro Materials and Devices 2011
EditorsSaulius Juodkazis, Min Gu
Place of PublicationBellingham WA USA
PublisherSPIE
Pages1 - 9
Number of pages9
Volume8204
ISBN (Print)9780819488459
DOIs
Publication statusPublished - 2011
Externally publishedYes
EventSPIE Smart Nano + Micro Materials and Devices 2011 - Swinburne University of Technology, Melbourne, Australia
Duration: 4 Dec 20117 Dec 2011
file:///C:/Users/annk/Downloads/AU11-final-L.pdf

Conference

ConferenceSPIE Smart Nano + Micro Materials and Devices 2011
CountryAustralia
CityMelbourne
Period4/12/117/12/11
Internet address

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