Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus: A functional imaging survey

Grégory Conductier, Agnès O. Martin, Pierre Yves Risold, Sonia Jego, Raphaël Lavoie, Chrystel Lafont, Patrice Mollard, Antoine Adamantidis, Jean-Louis Nahon

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.

Original languageEnglish
Article numberArticle 182
JournalFrontiers in Endocrinology
Volume4
Issue numberNOV
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Cilia
  • CSF flow
  • MCH
  • MCHR1
  • Non-neuronal function

Cite this

Conductier, Grégory ; Martin, Agnès O. ; Risold, Pierre Yves ; Jego, Sonia ; Lavoie, Raphaël ; Lafont, Chrystel ; Mollard, Patrice ; Adamantidis, Antoine ; Nahon, Jean-Louis. / Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus : A functional imaging survey. In: Frontiers in Endocrinology. 2013 ; Vol. 4, No. NOV.
@article{50b7d73d08c946169a6b60c31d174f25,
title = "Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus: A functional imaging survey",
abstract = "The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.",
keywords = "Cilia, CSF flow, MCH, MCHR1, Non-neuronal function",
author = "Gr{\'e}gory Conductier and Martin, {Agn{\`e}s O.} and Risold, {Pierre Yves} and Sonia Jego and Rapha{\"e}l Lavoie and Chrystel Lafont and Patrice Mollard and Antoine Adamantidis and Jean-Louis Nahon",
year = "2013",
doi = "10.3389/fendo.2013.00182",
language = "English",
volume = "4",
journal = "Frontiers in Endocrinology",
issn = "1664-2392",
publisher = "Frontiers Media",
number = "NOV",

}

Conductier, G, Martin, AO, Risold, PY, Jego, S, Lavoie, R, Lafont, C, Mollard, P, Adamantidis, A & Nahon, J-L 2013, 'Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus: A functional imaging survey', Frontiers in Endocrinology, vol. 4, no. NOV, Article 182. https://doi.org/10.3389/fendo.2013.00182

Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus : A functional imaging survey. / Conductier, Grégory; Martin, Agnès O.; Risold, Pierre Yves; Jego, Sonia; Lavoie, Raphaël; Lafont, Chrystel; Mollard, Patrice; Adamantidis, Antoine; Nahon, Jean-Louis.

In: Frontiers in Endocrinology, Vol. 4, No. NOV, Article 182, 2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus

T2 - A functional imaging survey

AU - Conductier, Grégory

AU - Martin, Agnès O.

AU - Risold, Pierre Yves

AU - Jego, Sonia

AU - Lavoie, Raphaël

AU - Lafont, Chrystel

AU - Mollard, Patrice

AU - Adamantidis, Antoine

AU - Nahon, Jean-Louis

PY - 2013

Y1 - 2013

N2 - The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.

AB - The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.

KW - Cilia

KW - CSF flow

KW - MCH

KW - MCHR1

KW - Non-neuronal function

UR - http://www.scopus.com/inward/record.url?scp=84890260631&partnerID=8YFLogxK

U2 - 10.3389/fendo.2013.00182

DO - 10.3389/fendo.2013.00182

M3 - Article

AN - SCOPUS:84890260631

VL - 4

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

SN - 1664-2392

IS - NOV

M1 - Article 182

ER -