Abstract
The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.
| Original language | English |
|---|---|
| Article number | Article 182 |
| Journal | Frontiers in Endocrinology |
| Volume | 4 |
| Issue number | NOV |
| DOIs | |
| Publication status | Published - 2013 |
| Externally published | Yes |
Keywords
- Cilia
- CSF flow
- MCH
- MCHR1
- Non-neuronal function
Cite this
}
Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus : A functional imaging survey. / Conductier, Grégory; Martin, Agnès O.; Risold, Pierre Yves; Jego, Sonia; Lavoie, Raphaël; Lafont, Chrystel; Mollard, Patrice; Adamantidis, Antoine; Nahon, Jean-Louis.
In: Frontiers in Endocrinology, Vol. 4, No. NOV, Article 182, 2013.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Control of ventricular ciliary beating by the melanin concentrating hormone-expressing neurons of the lateral hypothalamus
T2 - A functional imaging survey
AU - Conductier, Grégory
AU - Martin, Agnès O.
AU - Risold, Pierre Yves
AU - Jego, Sonia
AU - Lavoie, Raphaël
AU - Lafont, Chrystel
AU - Mollard, Patrice
AU - Adamantidis, Antoine
AU - Nahon, Jean-Louis
PY - 2013
Y1 - 2013
N2 - The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.
AB - The cyclic peptide Melanin Concentrating Hormone (MCH) is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory, and reward. Based on neuro-anatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (1) we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstrated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1-knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.
KW - Cilia
KW - CSF flow
KW - MCH
KW - MCHR1
KW - Non-neuronal function
UR - http://www.scopus.com/inward/record.url?scp=84890260631&partnerID=8YFLogxK
U2 - 10.3389/fendo.2013.00182
DO - 10.3389/fendo.2013.00182
M3 - Article
AN - SCOPUS:84890260631
VL - 4
JO - Frontiers in Endocrinology
JF - Frontiers in Endocrinology
SN - 1664-2392
IS - NOV
M1 - Article 182
ER -