TY - JOUR
T1 - Control of the Hippo pathway by Set7-dependent methylation of Yap
AU - Oudhoff, Menno J.
AU - Freeman, Spencer A.
AU - Couzens, Amber L.
AU - Antignano, Frann
AU - Kuznetsova, Ekaterina
AU - Min, Paul H.
AU - Northrop, Jeffrey P.
AU - Lehnertz, Bernhard
AU - Barsyte-Lovejoy, Dalia
AU - Vedadi, Masoud
AU - Arrowsmith, Cheryl H.
AU - Nishina, Hiroshi
AU - Gold, Michael R.
AU - Rossi, Fabio M.V.
AU - Gingras, Anne-Claude
AU - Zaph, Colby
PY - 2013/7/29
Y1 - 2013/7/29
N2 - Methylation of nonhistone proteins is emerging as a regulatory mechanism to control protein function. Set7 (Setd7) is a SET-domain-containing lysine methyltransferase that methylates and alters function of a variety of proteins in vitro, but the in vivo relevance has not been established. We found that Set7 is a modifier of the Hippo pathway. Mice that lack Set7 have a larger progenitor compartment in the intestine, coinciding with increased expression of Yes-associated protein (Yap) target genes. Mechanistically, monomethylation of lysine 494 of Yap is critical for cytoplasmic retention. These results identify a methylation-dependent checkpoint in the Hippo pathway.
AB - Methylation of nonhistone proteins is emerging as a regulatory mechanism to control protein function. Set7 (Setd7) is a SET-domain-containing lysine methyltransferase that methylates and alters function of a variety of proteins in vitro, but the in vivo relevance has not been established. We found that Set7 is a modifier of the Hippo pathway. Mice that lack Set7 have a larger progenitor compartment in the intestine, coinciding with increased expression of Yes-associated protein (Yap) target genes. Mechanistically, monomethylation of lysine 494 of Yap is critical for cytoplasmic retention. These results identify a methylation-dependent checkpoint in the Hippo pathway.
UR - http://www.scopus.com/inward/record.url?scp=84880945084&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2013.05.025
DO - 10.1016/j.devcel.2013.05.025
M3 - Article
C2 - 23850191
AN - SCOPUS:84880945084
SN - 1534-5807
VL - 26
SP - 188
EP - 194
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -