Control of attachment of Pseudomonas aeruginosa and Burkholderia cepacia to surfaces by shear force

Yew Woh Hui; Kumaran Narayanan; Gary A. Dykes

doi:10.2175/106143016X14504669767292

Control of attachment of Pseudomonas aeruginosa and Burkholderia cepacia to surfaces by shear force

Yew Woh Hui, Kumaran Narayanan, Gary A. Dykes

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflont was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm²) and B. cepacia (0.2-0.4 log CFU/cm²) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.

Original language	English
Pages (from-to)	2040-2046
Number of pages	7
Journal	Water Environment Research
Volume	88
Issue number	11
DOIs	https://doi.org/10.2175/106143016X14504669767292
Publication status	Published - 1 Nov 2016

Keywords

Attachment
Burkholderia cepacia
Cell surface
Pseudomonas aeruginosa
Shearing

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10.2175/106143016X14504669767292

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title = "Control of attachment of Pseudomonas aeruginosa and Burkholderia cepacia to surfaces by shear force",

abstract = "The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflont was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm2) and B. cepacia (0.2-0.4 log CFU/cm2) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.",

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author = "Hui, {Yew Woh} and Kumaran Narayanan and Dykes, {Gary A.}",

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AU - Hui, Yew Woh

AU - Narayanan, Kumaran

AU - Dykes, Gary A.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflont was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm2) and B. cepacia (0.2-0.4 log CFU/cm2) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.

AB - The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflont was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm2) and B. cepacia (0.2-0.4 log CFU/cm2) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.

KW - Attachment

KW - Burkholderia cepacia

KW - Cell surface

KW - Pseudomonas aeruginosa

KW - Shearing

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JO - Water Environment Research

JF - Water Environment Research

IS - 11

ER -

Control of attachment of Pseudomonas aeruginosa and Burkholderia cepacia to surfaces by shear force

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Keywords

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