Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats

Peng Zhou, Mary Tachedjian, James W. Wynne, Victoria Boyd, Jie Cui, Ina Smith, Christopher Cowled, Justin H. J. Ng, Laurence Mok, Wojtek P. Michalski, Ian H. Mendenhall, Gilda Tachedjian, Lin-Fa Wang, Michelle L. Baker

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Abstract

Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs of disease in bats. To determine the role of interferons (IFNs) in the ability of bats to coexist with viruses, we sequenced the type I IFN locus of the Australian black flying fox, Pteropus alecto, providing what is, to our knowledge, the first gene map of the IFN region of any bat species. Our results reveal a highly contracted type I IFN family consisting of only 10 IFNs, including three functional IFN-α loci. Furthermore, the three IFN-α genes are constitutively expressed in unstimulated bat tissues and cells and their expression is unaffected by viral infection. Constitutively expressed IFN-α results in the induction of a subset of IFN-stimulated genes associated with antiviral activity and resistance to DNA damage, providing evidence for a unique IFN system that may be linked to the ability of bats to coexist with viruses.
Original languageEnglish
Pages (from-to)2696-2701
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume113
Issue number10
DOIs
Publication statusPublished - 8 Mar 2016

Keywords

  • interferon
  • innate immunity
  • bat immunology

Cite this

Zhou, P., Tachedjian, M., Wynne, J. W., Boyd, V., Cui, J., Smith, I., ... Baker, M. L. (2016). Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats. Proceedings of the National Academy of Sciences, 113(10), 2696-2701. https://doi.org/10.1073/pnas.1518240113
Zhou, Peng ; Tachedjian, Mary ; Wynne, James W. ; Boyd, Victoria ; Cui, Jie ; Smith, Ina ; Cowled, Christopher ; Ng, Justin H. J. ; Mok, Laurence ; Michalski, Wojtek P. ; Mendenhall, Ian H. ; Tachedjian, Gilda ; Wang, Lin-Fa ; Baker, Michelle L. / Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats. In: Proceedings of the National Academy of Sciences. 2016 ; Vol. 113, No. 10. pp. 2696-2701.
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title = "Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats",
abstract = "Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs of disease in bats. To determine the role of interferons (IFNs) in the ability of bats to coexist with viruses, we sequenced the type I IFN locus of the Australian black flying fox, Pteropus alecto, providing what is, to our knowledge, the first gene map of the IFN region of any bat species. Our results reveal a highly contracted type I IFN family consisting of only 10 IFNs, including three functional IFN-α loci. Furthermore, the three IFN-α genes are constitutively expressed in unstimulated bat tissues and cells and their expression is unaffected by viral infection. Constitutively expressed IFN-α results in the induction of a subset of IFN-stimulated genes associated with antiviral activity and resistance to DNA damage, providing evidence for a unique IFN system that may be linked to the ability of bats to coexist with viruses.",
keywords = "interferon, innate immunity, bat immunology",
author = "Peng Zhou and Mary Tachedjian and Wynne, {James W.} and Victoria Boyd and Jie Cui and Ina Smith and Christopher Cowled and Ng, {Justin H. J.} and Laurence Mok and Michalski, {Wojtek P.} and Mendenhall, {Ian H.} and Gilda Tachedjian and Lin-Fa Wang and Baker, {Michelle L.}",
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doi = "10.1073/pnas.1518240113",
language = "English",
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Zhou, P, Tachedjian, M, Wynne, JW, Boyd, V, Cui, J, Smith, I, Cowled, C, Ng, JHJ, Mok, L, Michalski, WP, Mendenhall, IH, Tachedjian, G, Wang, L-F & Baker, ML 2016, 'Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats', Proceedings of the National Academy of Sciences, vol. 113, no. 10, pp. 2696-2701. https://doi.org/10.1073/pnas.1518240113

Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats. / Zhou, Peng; Tachedjian, Mary; Wynne, James W.; Boyd, Victoria; Cui, Jie; Smith, Ina; Cowled, Christopher; Ng, Justin H. J.; Mok, Laurence; Michalski, Wojtek P.; Mendenhall, Ian H.; Tachedjian, Gilda; Wang, Lin-Fa; Baker, Michelle L.

In: Proceedings of the National Academy of Sciences, Vol. 113, No. 10, 08.03.2016, p. 2696-2701.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats

AU - Zhou, Peng

AU - Tachedjian, Mary

AU - Wynne, James W.

AU - Boyd, Victoria

AU - Cui, Jie

AU - Smith, Ina

AU - Cowled, Christopher

AU - Ng, Justin H. J.

AU - Mok, Laurence

AU - Michalski, Wojtek P.

AU - Mendenhall, Ian H.

AU - Tachedjian, Gilda

AU - Wang, Lin-Fa

AU - Baker, Michelle L.

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N2 - Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs of disease in bats. To determine the role of interferons (IFNs) in the ability of bats to coexist with viruses, we sequenced the type I IFN locus of the Australian black flying fox, Pteropus alecto, providing what is, to our knowledge, the first gene map of the IFN region of any bat species. Our results reveal a highly contracted type I IFN family consisting of only 10 IFNs, including three functional IFN-α loci. Furthermore, the three IFN-α genes are constitutively expressed in unstimulated bat tissues and cells and their expression is unaffected by viral infection. Constitutively expressed IFN-α results in the induction of a subset of IFN-stimulated genes associated with antiviral activity and resistance to DNA damage, providing evidence for a unique IFN system that may be linked to the ability of bats to coexist with viruses.

AB - Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs of disease in bats. To determine the role of interferons (IFNs) in the ability of bats to coexist with viruses, we sequenced the type I IFN locus of the Australian black flying fox, Pteropus alecto, providing what is, to our knowledge, the first gene map of the IFN region of any bat species. Our results reveal a highly contracted type I IFN family consisting of only 10 IFNs, including three functional IFN-α loci. Furthermore, the three IFN-α genes are constitutively expressed in unstimulated bat tissues and cells and their expression is unaffected by viral infection. Constitutively expressed IFN-α results in the induction of a subset of IFN-stimulated genes associated with antiviral activity and resistance to DNA damage, providing evidence for a unique IFN system that may be linked to the ability of bats to coexist with viruses.

KW - interferon

KW - innate immunity

KW - bat immunology

UR - http://www.ncbi.nlm.nih.gov/pubmed/26903655

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DO - 10.1073/pnas.1518240113

M3 - Article

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SP - 2696

EP - 2701

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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