Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor

The roles of chem. kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quant. comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amts. of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyzes the oxidn. of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochem. redn. back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the crit. effect of substrate and cosubstrate concn. on amperometric immunosensors construction in which HRP is used as an enzymic label was studied. [on SciFinder (R)]