TY - JOUR
T1 - Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor
AU - Salinas, Eloy
AU - Torriero, Angel Alberto Jesus
AU - Sanz-Ferramola, Maria Isabel
AU - Battaglini, Fernando
AU - Raba, Julio
PY - 2005
Y1 - 2005
N2 - The roles of chem. kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quant. comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amts. of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyzes the oxidn. of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochem. redn. back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the crit. effect of substrate and cosubstrate concn. on amperometric immunosensors construction in which HRP is used as an enzymic label was studied. [on SciFinder (R)]
AB - The roles of chem. kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quant. comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amts. of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyzes the oxidn. of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochem. redn. back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the crit. effect of substrate and cosubstrate concn. on amperometric immunosensors construction in which HRP is used as an enzymic label was studied. [on SciFinder (R)]
UR - http://www.sciencedirect.com.ezproxy.lib.monash.edu.au/science?_ob=PublicationURL&_tockey=%23TOC%235288%232005%23999339998%23592283%23FLA%23&_cdi=5288
M3 - Article
VL - 66
SP - 92
EP - 102
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
ER -