Projects per year
Abstract
Grb7 is an adapter protein, aberrantly co-overexpressed with HER2 and identified as an independent prognostic marker in breast cancer. It has been established that Grb7 exacerbates the cellular growth and migratory behaviour of HER2+ve breast cancer cells. Less is known about Grb7 s role in the context of HER2-ve cells. Here we directly compare the effect of stable Grb7 knockdown in oestrogen sensitive (T47D), HER2+ve (SKBR3) and triple-negative (MDA-MB-468 and MDA-MB-231) breast cancer cell lines on anchorage dependent and independent cell growth, wound healing and chemotaxis. All cell lines showed reduced ability to migrate upon Grb7 knockdown, despite their greatly varied endogenous levels of Grb7. Decreased cell proliferation was not observed in any of the cell lines upon Grb7 knockdown; however, decreased ability to form colonies was observed for all but the oestrogen sensitive cell line, depending upon the stringency of the growth conditions. The data reveal that Grb7 plays an important role in breast cancer progression, beyond the context of HER2+ve cell types.
Original language | English |
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Pages (from-to) | 593 - 603 |
Number of pages | 11 |
Journal | Breast Cancer Research and Treatment |
Volume | 143 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2014 |
Projects
- 2 Finished
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Characterisation of the growth receptor bound 7 (Grb7) protein and protein and RNA partners involved in the regulation of stress granule formation and cell migration.
Wilce, J., Price, J. & Gorospe, M.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/11 → 31/12/13
Project: Research
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Identification and characterization of novel molecular modulators of tumour cell metastasis
Price, J.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/06 → 31/12/10
Project: Research