Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant

Nick Dorrell, Shu Rui Li, Pau H. Everest, Gordon Dougan, Brendan W. Wren

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49 Citations (Scopus)

Abstract

The ompR-envZ two-component regulatory system has been shown to contribute to virulence in a number of enteric bacterial pathogens. A Yersinia enterocolitica O:8 ompR homologue was amplified, cloned and sequenced, showing 99.2% homology to the Escherichia coli OmpR. An isogenic ompR mutant was constructed by reverse genetics-based methodology. The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalFEMS Microbiology Letters
Volume165
Issue number1
DOIs
Publication statusPublished - 1 Aug 1998
Externally publishedYes

Keywords

  • Isogenic mutant
  • OmpR
  • Pathogenesis
  • Yersinia enterocolitica

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