Constitutive Expression of a 2′,5′-Oligoadenylate Synthetase cDNA Results in Increased Antiviral Activity and Growth Suppression

Gillian Rysiecki, Dirk R. Gewert, Bryan R.g. Williams

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Abstract

The interferon (IFN)-induced enzyme 2′,5′-oligoadeny late (2-5A) synthetase has been implicated in the development of antiviral activity in human and animal cells. However, its role in IFN-mediated growth inhibition remains unclear. To elucidate the function of 2-5A synthetase, we have stably introduced a human 2-5A synthetase cDNA into a human glioblastoma cell line (T98G). Constitutive expression of the cDNA in these cells is associated with increased levels of resistance to infection by encephalomyocarditis virus. One transfected subclone, which expresses elevated levels of 2-5A synthetase enzyme activity, also shows a reduced rate of cellular proliferation.

Original languageEnglish
Pages (from-to)649-657
Number of pages9
JournalJournal of Interferon Research
Volume9
Issue number6
DOIs
Publication statusPublished - Dec 1989
Externally publishedYes

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