Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells

Sanja Coso, Yiping Zeng, Dhanya Sreedharan, Elizabeth Williams

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function.
Original languageEnglish
Pages (from-to)2397 - 2407
Number of pages11
JournalExperimental Cell Research
Volume317
Issue number17
DOIs
Publication statusPublished - 2011

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