Consensus protein design

Benjamin T. Porebski, Ashley M. Buckle

Research output: Contribution to journalReview ArticleResearchpeer-review

52 Citations (Scopus)


A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved amino acids. Here, we review the consensus design approach, its theoretical underpinnings, successes, limitations and challenges, as well as providing a detailed guide to its application in protein engineering.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalProtein Engineering Design and Selection
Issue number7
Publication statusPublished - 1 Jul 2016


  • consensus design
  • multiple sequence alignment
  • protein stability
  • semi-rational design
  • statistical sequence analysis
  • thermostability

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