Abstract
A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved amino acids. Here, we review the consensus design approach, its theoretical underpinnings, successes, limitations and challenges, as well as providing a detailed guide to its application in protein engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 245-251 |
| Number of pages | 7 |
| Journal | Protein Engineering Design and Selection |
| Volume | 29 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2016 |
Keywords
- consensus design
- multiple sequence alignment
- protein stability
- semi-rational design
- statistical sequence analysis
- thermostability