Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency

Karthikeyan Gunasekaran, Thi Hoa Tam Nguyen, Heather D Maynard, Thomas Paul Davis, Volga Bulmus

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

A thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56? 5 -58? 3 of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77? 7 ).
Original languageEnglish
Pages (from-to)654 - 659
Number of pages6
JournalMacromolecular Rapid Communications
Volume32
Issue number8
DOIs
Publication statusPublished - 2011
Externally publishedYes

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