TY - JOUR
T1 - Computer-aided three-dimensional reconstruction of nematode embryos from EM serial sections
AU - Schierenberg, Einhard
AU - Cole, Tom
AU - Carlson, Christopher
AU - Sidio, Wayne
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Embryos of the nematode Caenorhabditis elegans were serially sectioned and photographed in the electron microscope (EM). The micrographs were used to produce threedimensional (3D) reconstructions. Size and position of each nucleus were entered into a computer, displayed as spheres, and were color-coded to indicate lineage membership. Location in space and position in the cell cycle are generally adequate criteria to identify cells. The reconstructions allow visualization of lineage-related topographic patterns and ultrastructural analysis of differently determined cells.
AB - Embryos of the nematode Caenorhabditis elegans were serially sectioned and photographed in the electron microscope (EM). The micrographs were used to produce threedimensional (3D) reconstructions. Size and position of each nucleus were entered into a computer, displayed as spheres, and were color-coded to indicate lineage membership. Location in space and position in the cell cycle are generally adequate criteria to identify cells. The reconstructions allow visualization of lineage-related topographic patterns and ultrastructural analysis of differently determined cells.
UR - http://www.scopus.com/inward/record.url?scp=0022504217&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(86)90524-0
DO - 10.1016/0014-4827(86)90524-0
M3 - Article
C2 - 3743656
AN - SCOPUS:0022504217
SN - 0014-4827
VL - 166
SP - 247
EP - 252
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -