Comprehensive two-dimensional liquid chromatography for polyphenol analysis in foodstuffs

Francesco Cacciola, Sara Farnetti, Paola Dugo, Philip Marriott, Luigi Mondello

Research output: Contribution to journalReview ArticleResearchpeer-review

34 Citations (Scopus)


Polyphenols are a class of plant secondary metabolites that are recently drawing a special interest because of their broad spectrum of pharmacological effects. As they are characterized by an enormous structural variability, the identification of these molecules in food samples is a difficult task, and sometimes having only a limited number of commercially available reference materials is not of great help. One-dimensional liquid chromatography is the most widely applied analytical approach for their analysis. In particular, the hyphenation of liquid chromatography to mass spectrometry has come to play an influential role by allowing relatively fast tentative identification and accurate quantification of polyphenolic compounds at trace levels in vegetable media. However, when dealing with very complex real-world food samples, a single separation system often does not provide sufficient resolving power for attaining rewarding results. Comprehensive two-dimensional liquid chromatography is a technique of great analytical impact, since it offers much higher peak capacities than separations in a single dimension. In the present review, we describe applications in the field of comprehensive two-dimensional liquid chromatography for polyphenol analysis in real-world food samples. Comprehensive two-dimensional liquid chromatography applications to nonfood matrices fall outside the scope of the current report and will not be discussed.

Original languageEnglish
Pages (from-to)7-24
Number of pages18
JournalJournal of Separation Science
Issue number1
Publication statusPublished - 2017


  • Comprehensive two-dimensional liquid chromatography
  • Food
  • Mass spectrometry
  • Polyphenols

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