Comprehensive two-dimensional gas chromatography (GC × GC) and GC × GC-quadrupole MS analysis of Asian and American ginseng

Robert A. Shellie, Philip J. Marriott, Carmen W. Huie

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74 Citations (Scopus)

Abstract

Extracts of the roots of Panax (ginseng) species are analysed using comprehensive two-dimensional gas chromatography. Comparison of comprehensive two-dimensional chromatograms reveals the presence of numerous common components, and possible species-specific components are also observed. Two-dimensional bubble plot presentations of GC × GC(FID) data allow ready qualitative and semi-quantitative comparison of the extracts. The use of GC × GC-quadrupole MS (GC × GC-qMS), operated at a scanning rate of 20 Hz, in conjunction with retention data, permits identification of separated (major) components. Whilst this scan speed will be insufficient for reliable quantitative analysis, considerably improved quality of uncorrected spectra, arising from the enhanced separation over single column GC-MS analysis is apparent. Spectral match qualities of up to 99% are found for a number of components. Comparison of GC × GC-qMS with GC × GC(FID) indicates that good correspondence of peak position patterns is achieved, which aids component identification. It is shown that (quadrupole) mass spectrometric detection is a useful tool for provision of identification of separated compounds in high-resolution comprehensive two-dimensional gas chromatography.

Original languageEnglish
Pages (from-to)1185-1192
Number of pages8
JournalJournal of Separation Science
Volume26
Issue number12-13
DOIs
Publication statusPublished - 1 Aug 2003
Externally publishedYes

Keywords

  • Comprehensive gas chromatography
  • GC × GC-MS
  • Mass spectrometry
  • Panax ginseng
  • Panax notoginseng
  • Panax quinquefolium

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