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Comprehensive Targeted Lipidomic Profiling for Research and Clinical Applications

  • Kevin Huynh
  • , Thy Duong
  • , Natalie A. Mellett
  • , Michelle Cinel
  • , Corey Giles
  • , Peter J. Meikle

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Mass spectrometry remains one of the gold standard approaches in examining the lipidome in biological samples. Recently, advancements in chromatography and mass spectrometry approaches have enabled broad coverage of the lipidome. However, many limitations still exist, and lipidomic analysis often requires a fine balance between coverage of the lipidome, structural detail, and sample throughput. For biomedical and clinical research using human samples, the diversity and natural variation between different individuals necessitate larger sample numbers to identify significant associations with clinical outcomes and account for potential confounding factors. Here we describe a targeted lipidomics workflow that enables reproducible profiling of thousands of plasma samples in a systematic manner, while maintaining good structural detail and high coverage of the lipidome.

Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsDavid W. Greening, Richard J. Simpson
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter29
Pages489-504
Number of pages16
Edition3rd
ISBN (Electronic)9781071629789
ISBN (Print)9781071629772
DOIs
Publication statusPublished - 2023
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2628
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cohort studies
  • Lipidomics
  • Mass spectrometry
  • Plasma
  • Targeted lipidomics

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