Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Xiaodong Liu, Christian M Nefzger, Fernando J Rossello, Joseph Chen, Anja S Knaupp, Jaber Firas, Ethan Ford, Jahnvi Pflueger, Jacob M Paynter, Hun S Chy, Carmel M O'Brien, Cheng Huang, Ketan Mishra, Margeaux Hodgson-Garms, Natasha Jansz, Sarah M Williams, Marnie E Blewitt, Susan K Nilsson, Ralf B Schittenhelm, Andrew L Laslett & 2 others Ryan Lister, Jose M Polo

Research output: Research - peer-reviewArticle

Abstract

Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.

LanguageEnglish
Pages1055-1062
Number of pages8
JournalNature Methods
Volume14
Issue number11
DOIs
StatePublished - 31 Oct 2017

Keywords

  • biological models
  • embryonic stem cells
  • pluripotent stem cells
  • reprogramming

Cite this

Liu, Xiaodong ; Nefzger, Christian M ; Rossello, Fernando J ; Chen, Joseph ; Knaupp, Anja S ; Firas, Jaber ; Ford, Ethan ; Pflueger, Jahnvi ; Paynter, Jacob M ; Chy, Hun S ; O'Brien, Carmel M ; Huang, Cheng ; Mishra, Ketan ; Hodgson-Garms, Margeaux ; Jansz, Natasha ; Williams, Sarah M ; Blewitt, Marnie E ; Nilsson, Susan K ; Schittenhelm, Ralf B ; Laslett, Andrew L ; Lister, Ryan ; Polo, Jose M. / Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming. In: Nature Methods. 2017 ; Vol. 14, No. 11. pp. 1055-1062
@article{6021491e00a741539efb9cb301f2e765,
title = "Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming",
abstract = "Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.",
keywords = "biological models, embryonic stem cells, pluripotent stem cells, reprogramming",
author = "Xiaodong Liu and Nefzger, {Christian M} and Rossello, {Fernando J} and Joseph Chen and Knaupp, {Anja S} and Jaber Firas and Ethan Ford and Jahnvi Pflueger and Paynter, {Jacob M} and Chy, {Hun S} and O'Brien, {Carmel M} and Cheng Huang and Ketan Mishra and Margeaux Hodgson-Garms and Natasha Jansz and Williams, {Sarah M} and Blewitt, {Marnie E} and Nilsson, {Susan K} and Schittenhelm, {Ralf B} and Laslett, {Andrew L} and Ryan Lister and Polo, {Jose M}",
year = "2017",
month = "10",
doi = "10.1038/nmeth.4436",
volume = "14",
pages = "1055--1062",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "11",

}

Liu, X, Nefzger, CM, Rossello, FJ, Chen, J, Knaupp, AS, Firas, J, Ford, E, Pflueger, J, Paynter, JM, Chy, HS, O'Brien, CM, Huang, C, Mishra, K, Hodgson-Garms, M, Jansz, N, Williams, SM, Blewitt, ME, Nilsson, SK, Schittenhelm, RB, Laslett, AL, Lister, R & Polo, JM 2017, 'Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming' Nature Methods, vol 14, no. 11, pp. 1055-1062. DOI: 10.1038/nmeth.4436

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming. / Liu, Xiaodong; Nefzger, Christian M; Rossello, Fernando J; Chen, Joseph; Knaupp, Anja S; Firas, Jaber; Ford, Ethan; Pflueger, Jahnvi; Paynter, Jacob M; Chy, Hun S; O'Brien, Carmel M; Huang, Cheng; Mishra, Ketan; Hodgson-Garms, Margeaux; Jansz, Natasha; Williams, Sarah M; Blewitt, Marnie E; Nilsson, Susan K; Schittenhelm, Ralf B; Laslett, Andrew L; Lister, Ryan; Polo, Jose M.

In: Nature Methods, Vol. 14, No. 11, 31.10.2017, p. 1055-1062.

Research output: Research - peer-reviewArticle

TY - JOUR

T1 - Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

AU - Liu,Xiaodong

AU - Nefzger,Christian M

AU - Rossello,Fernando J

AU - Chen,Joseph

AU - Knaupp,Anja S

AU - Firas,Jaber

AU - Ford,Ethan

AU - Pflueger,Jahnvi

AU - Paynter,Jacob M

AU - Chy,Hun S

AU - O'Brien,Carmel M

AU - Huang,Cheng

AU - Mishra,Ketan

AU - Hodgson-Garms,Margeaux

AU - Jansz,Natasha

AU - Williams,Sarah M

AU - Blewitt,Marnie E

AU - Nilsson,Susan K

AU - Schittenhelm,Ralf B

AU - Laslett,Andrew L

AU - Lister,Ryan

AU - Polo,Jose M

PY - 2017/10/31

Y1 - 2017/10/31

N2 - Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.

AB - Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.

KW - biological models

KW - embryonic stem cells

KW - pluripotent stem cells

KW - reprogramming

UR - http://www.scopus.com/inward/record.url?scp=85032566101&partnerID=8YFLogxK

U2 - 10.1038/nmeth.4436

DO - 10.1038/nmeth.4436

M3 - Article

VL - 14

SP - 1055

EP - 1062

JO - Nature Methods

T2 - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 11

ER -