Projects per year
Abstract
Antimicrobial resistance (AMR) continues to evolve as a major threat to human health, and new strategies are required for the treatment of AMR infections. Bacteriophages (phages) that kill bacterial pathogens are being identified for use in phage therapies, with the intention to apply these bactericidal viruses directly into the infection sites in bespoke phage cocktails. Despite the great unsampled phage diversity for this purpose, an issue hampering the roll out of phage therapy is the poor quality annotation of many of the phage genomes, particularly for those from infrequently sampled environmental sources. We developed a computational tool called STEP3 to use the "evolutionary features" that can be recognized in genome sequences of diverse phages. These features, when integrated into an ensemble framework, achieved a stable and robust prediction performance when benchmarked against other prediction tools using phages from diverse sources. Validation of the prediction accuracy of STEP3 was conducted with high-resolution mass spectrometry analysis of two novel phages, isolated from a watercourse in the Southern Hemisphere. STEP3 provides a robust computational approach to distinguish specific and universal features in phages to improve the quality of phage cocktails and is available for use at http://step3.erc.monash.edu/.
Original language | English |
---|---|
Article number | e00242-21 |
Number of pages | 17 |
Journal | mSystems |
Volume | 6 |
Issue number | 3 |
DOIs | |
Publication status | Published - 27 May 2021 |
Keywords
- Antimicrobial resistance
- Artificial intelligence
- Bacteriophage
- Bacteriophage therapy
- Bacteriophages
- Klebsiella
- Machine learning
- Phage therapy
- Virion structure
Projects
- 1 Finished
-
Molecular Machines and Bacterial Cell Biology.
Australian Research Council (ARC)
31/12/13 → 16/07/19
Project: Research
Equipment
-
Monash Proteomics & Metabolomics Facility
Ralf Schittenhelm (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility