Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells

Ana Maria Valencia-Hernandez; Thomas Zillinger; Zhengyu Ge; Peck S. Tan; Anton Cozijnsen; Geoffrey I. McFadden; Mireille H. Lahoud; Irina Caminschi; Winfried Barchet; William R. Heath; Daniel Fernandez-Ruiz

doi:10.1016/j.vaccine.2022.12.047

Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver T_RM cells

Ana Maria Valencia-Hernandez, Thomas Zillinger, Zhengyu Ge, Peck S. Tan, Anton Cozijnsen, Geoffrey I. McFadden, Mireille H. Lahoud, Irina Caminschi, Winfried Barchet, William R. Heath, Daniel Fernandez-Ruiz

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Tissue resident memory T cells (T_RM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating T_RM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8⁺ T_RM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8⁺ T_RM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver T_RM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver T_RM-based vaccines for their potential translation.

Original language	English
Pages (from-to)	1094-1107
Number of pages	14
Journal	Vaccine
Volume	41
Issue number	5
DOIs	https://doi.org/10.1016/j.vaccine.2022.12.047
Publication status	Published - 27 Jan 2023

Keywords

Adjuvants
Liver immunology
Pattern recognition receptors
T cell memory
Tissue-resident memory T cells
Toll-like receptors
type I dendritic cells
Vaccination

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vaccine.2022.12.047

Cite this

@article{cdb4bbc1ce0c407eb02c93be8ee1f919,

title = "Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells",

abstract = "Tissue resident memory T cells (TRM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver TRM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver TRM-based vaccines for their potential translation.",

keywords = "Adjuvants, Liver immunology, Pattern recognition receptors, T cell memory, Tissue-resident memory T cells, Toll-like receptors, type I dendritic cells, Vaccination",

author = "Valencia-Hernandez, {Ana Maria} and Thomas Zillinger and Zhengyu Ge and Tan, {Peck S.} and Anton Cozijnsen and {I. McFadden}, Geoffrey and Lahoud, {Mireille H.} and Irina Caminschi and Winfried Barchet and Heath, {William R.} and Daniel Fernandez-Ruiz",

note = "Funding Information: We thank Melanie Damtsis and Ming Li for technical assistance, and the members of the W.R.H., L.K.M. and S.M. labs for discussion and the Doherty{\textquoteright}s animal facility staff for mice husbandry. This work was supported by the National Health and Medical Research Council of Australia; D.F.R (NHMRC 1139486), W.R.H. (NHMRC 1154457, NHMRC 1113293, NHMRC 1124706), I.C. (NHMRC 1124706). W.B. was supported by the German Center for Infection Research (DZIF: TTU 09.702) and the German Research Foundation (DFG: IRTG 2168). Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2023",

month = jan,

day = "27",

doi = "10.1016/j.vaccine.2022.12.047",

language = "English",

volume = "41",

pages = "1094--1107",

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T1 - Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells

AU - Valencia-Hernandez, Ana Maria

AU - Zillinger, Thomas

AU - Ge, Zhengyu

AU - Tan, Peck S.

AU - Cozijnsen, Anton

AU - I. McFadden, Geoffrey

AU - Lahoud, Mireille H.

AU - Caminschi, Irina

AU - Barchet, Winfried

AU - Heath, William R.

AU - Fernandez-Ruiz, Daniel

N1 - Funding Information: We thank Melanie Damtsis and Ming Li for technical assistance, and the members of the W.R.H., L.K.M. and S.M. labs for discussion and the Doherty’s animal facility staff for mice husbandry. This work was supported by the National Health and Medical Research Council of Australia; D.F.R (NHMRC 1139486), W.R.H. (NHMRC 1154457, NHMRC 1113293, NHMRC 1124706), I.C. (NHMRC 1124706). W.B. was supported by the German Center for Infection Research (DZIF: TTU 09.702) and the German Research Foundation (DFG: IRTG 2168). Publisher Copyright: © 2022 Elsevier Ltd

PY - 2023/1/27

Y1 - 2023/1/27

N2 - Tissue resident memory T cells (TRM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver TRM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver TRM-based vaccines for their potential translation.

AB - Tissue resident memory T cells (TRM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver TRM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver TRM-based vaccines for their potential translation.

KW - Adjuvants

KW - Liver immunology

KW - Pattern recognition receptors

KW - T cell memory

KW - Tissue-resident memory T cells

KW - Toll-like receptors

KW - type I dendritic cells

KW - Vaccination

UR - http://www.scopus.com/inward/record.url?scp=85147204285&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2022.12.047

DO - 10.1016/j.vaccine.2022.12.047

M3 - Article

C2 - 36609029

AN - SCOPUS:85147204285

SN - 0264-410X

VL - 41

SP - 1094

EP - 1107

JO - Vaccine

JF - Vaccine

IS - 5

ER -

Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver T_RM cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Protecting against malaria through liver-resident memory T cells

Targeting antigen to Clec9A on dendritic cell for humoral immunity

Cite this

Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Projects

Protecting against malaria through liver-resident memory T cells

Targeting antigen to Clec9A on dendritic cell for humoral immunity

Cite this

Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver T_RM cells