Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparum PfRh4 invasion ligand

Wai Hong Tham, Danny W. Wilson, Sash Lopaticki, Christoph Q. Schmidt, Patience B. Tetteh-Quarcoo, Paul N. Barlow, Dave Richard, Jason E. Corbin, James G. Beeson, Alan F. Cowman

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145 Citations (Scopus)

Abstract

Plasmodium falciparum is responsible for the most severe form of malaria disease in humans, causing more than 1 million deaths each year. As an obligate intracellular parasite, P. falciparum's ability to invade erythrocytes is essential for its survival within the human host. P. falciparum invades erythrocytes using multiple host receptor-parasite ligand interactions known as invasion pathways. Here weshow that CR1 is the host erythrocyte receptor for PfRh4, a major P. falciparum ligand essential for sialic acid-independent invasion. PfRh4 and CR1 interact directly, with a Kd of 2.9 μM. PfRh4 binding is strongly correlated with the CR1 level on the erythrocyte surface. Parasite invasion via sialic acid-independent pathways is reduced in low-CR1 erythrocytes due to limited availability of this receptor on the surface. Furthermore, soluble CR1 can competitively block binding of PfRh4 to the erythrocyte surface and specifically inhibit sialic acid-independent parasite invasion. These results demonstrate that CR1 is an erythrocyte receptor used by the parasite ligand PfRh4 for P. falciparum invasion.

Original languageEnglish
Pages (from-to)17327-17332
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number40
DOIs
Publication statusPublished - 5 Oct 2010
Externally publishedYes

Keywords

  • Malaria
  • Merozoite
  • Red blood cell
  • Reticulocyte-binding-like homologue

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