Compartmentalizing genetically encoded calcium sensors

David A. Williams, Mastura Monif, Kate L. Richardson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

Within single cells there is a complex myriad of signaling which controls physiological process many of which are modulated, or signaled directly, by intracellular calcium ions. Understanding the exquisitely sensitive, and spatially restricted, changes in calcium has been of interest to the researcher for a number of years. Recent advances in thisfield have been driven by the development of genetically encoded calcium probes for detecting calcium changes within the cells specifically targeting organelles such as mitochondria, endoplasmic reticulum, and the nucleus. In this chapter the authors outline some of the available fluorescent probes, with particular emphasis on an endoplasmic reticulum targeted calcium biosensor in cell signaling studies with astrocytes, detailing experimental protocols and the interpretation of data from such probes.

Original languageEnglish
Title of host publicationCalcium Signaling Protocols
EditorsDavid G. Lambert, Richard D. Rainbow
Place of PublicationTotowa, NJ
PublisherHumana Press
Chapter19
Pages307-326
Number of pages20
EditionThird
ISBN (Electronic)9781627030861
ISBN (Print)9781627030854
DOIs
Publication statusPublished - 1 Jan 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume937
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Astrocytes
  • Calcium biosensor
  • Confocal microscopy
  • D1-ER cameleon
  • Endoplasmic reticulum
  • Transfection

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