Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

Jeonghun Baek; Chun Wai Mai; Wei Meng Lim; Lai Chun Wong

doi:10.18240/ijo.2023.01.06

Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

Jeonghun Baek, Chun Wai Mai, Wei Meng Lim, Lai Chun Wong

Research output: Contribution to journal › Article › Research › peer-review

Abstract

AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro.

METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO₂ incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography.

RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil.

CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

Original language	English
Pages (from-to)	40-46
Number of pages	7
Journal	International Journal of Ophthalmology
Volume	16
Issue number	1
DOIs	https://doi.org/10.18240/ijo.2023.01.06
Publication status	Published - 18 Jan 2023
Externally published	Yes

Keywords

adult retinal pigment epithelial-19 cells
lutein
vegetable oil
zeaxanthin

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title = "Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells",

abstract = "AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO2 incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography. RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil. CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.",

keywords = "adult retinal pigment epithelial-19 cells, lutein, vegetable oil, zeaxanthin",

author = "Jeonghun Baek and Mai, {Chun Wai} and Lim, {Wei Meng} and Wong, {Lai Chun}",

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doi = "10.18240/ijo.2023.01.06",

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AU - Baek, Jeonghun

AU - Mai, Chun Wai

AU - Lim, Wei Meng

AU - Wong, Lai Chun

PY - 2023/1/18

Y1 - 2023/1/18

N2 - AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO2 incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography. RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil. CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

AB - AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO2 incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography. RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil. CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

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Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

Abstract

Keywords

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