Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma

Mehrdad Nikfarjam, Dannel Yeo, Hong He, Graham Baldwin, Theodora Fifis, Patricia Costa, Bryan Tan, Eunice Yang, Shu W. Wen, Christopher Christophi

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Background: Pancreatic adenocarcinoma has an extremely poor prognosis. The use of appropriate in vivo models is essential in devising methods to improve treatment outcomes. Methods: A pancreatic adenocarcinoma model based on tumor injection into the pancreatic head was compared with a pancreatic tail injection model in C57/BL6 mice. The murine pancreatic adenocarcinoma cell line PAN02, dispersed in MatrigelTM, was used for tumor induction. Results: Tumors developed in all animals in both models. Tumor size was more consistent within the pancreatic tail group at 20 days following induction, with no evidence of metastatic disease. Animals in the pancreatic head injection group showed signs of reduced health by 20 days following injection and developed jaundice. Microscopic liver metastases were noted in some of these animals at this time point. The overall survival of animals at 40 days following tumor induction was significantly lower in the pancreatic head injection group (0% vs. 35%; p < .001). Multiple liver metastases were noted in five of 10 (50%) animals in the head injection group, without evidence of peritoneal metastases. In the pancreatic tail injection group, 18 of 20 (90%) animals had multiple peritoneal metastases, and nine of 20 (45%) animals had evidence of isolated liver deposits. Tumors in both regions of the pancreas had similar histologic characteristics, with a dense fibrotic stroma at the interface between the tumor and the normal pancreas. Conclusion: Pancreatic head and tail orthotopic cancer models produce consistent tumors, but the patterns of tumor spread and survival differ according to the site of injection. 

Original languageEnglish
Pages (from-to)352-359
Number of pages8
JournalJournal of Investigative Surgery
Volume26
Issue number6
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

Keywords

  • Animal models
  • Metastatic neoplasm
  • Pancreatic neoplasm

Cite this

Nikfarjam, Mehrdad ; Yeo, Dannel ; He, Hong ; Baldwin, Graham ; Fifis, Theodora ; Costa, Patricia ; Tan, Bryan ; Yang, Eunice ; Wen, Shu W. ; Christophi, Christopher. / Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma. In: Journal of Investigative Surgery. 2013 ; Vol. 26, No. 6. pp. 352-359.
@article{7dde82d9c39941c3b6c75074cbef87c3,
title = "Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma",
abstract = "Background: Pancreatic adenocarcinoma has an extremely poor prognosis. The use of appropriate in vivo models is essential in devising methods to improve treatment outcomes. Methods: A pancreatic adenocarcinoma model based on tumor injection into the pancreatic head was compared with a pancreatic tail injection model in C57/BL6 mice. The murine pancreatic adenocarcinoma cell line PAN02, dispersed in MatrigelTM, was used for tumor induction. Results: Tumors developed in all animals in both models. Tumor size was more consistent within the pancreatic tail group at 20 days following induction, with no evidence of metastatic disease. Animals in the pancreatic head injection group showed signs of reduced health by 20 days following injection and developed jaundice. Microscopic liver metastases were noted in some of these animals at this time point. The overall survival of animals at 40 days following tumor induction was significantly lower in the pancreatic head injection group (0{\%} vs. 35{\%}; p < .001). Multiple liver metastases were noted in five of 10 (50{\%}) animals in the head injection group, without evidence of peritoneal metastases. In the pancreatic tail injection group, 18 of 20 (90{\%}) animals had multiple peritoneal metastases, and nine of 20 (45{\%}) animals had evidence of isolated liver deposits. Tumors in both regions of the pancreas had similar histologic characteristics, with a dense fibrotic stroma at the interface between the tumor and the normal pancreas. Conclusion: Pancreatic head and tail orthotopic cancer models produce consistent tumors, but the patterns of tumor spread and survival differ according to the site of injection. ",
keywords = "Animal models, Metastatic neoplasm, Pancreatic neoplasm",
author = "Mehrdad Nikfarjam and Dannel Yeo and Hong He and Graham Baldwin and Theodora Fifis and Patricia Costa and Bryan Tan and Eunice Yang and Wen, {Shu W.} and Christopher Christophi",
year = "2013",
month = "12",
doi = "10.3109/08941939.2013.797057",
language = "English",
volume = "26",
pages = "352--359",
journal = "Journal of Investigative Surgery",
issn = "0894-1939",
publisher = "Taylor & Francis",
number = "6",

}

Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma. / Nikfarjam, Mehrdad; Yeo, Dannel; He, Hong; Baldwin, Graham; Fifis, Theodora; Costa, Patricia; Tan, Bryan; Yang, Eunice; Wen, Shu W.; Christophi, Christopher.

In: Journal of Investigative Surgery, Vol. 26, No. 6, 12.2013, p. 352-359.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma

AU - Nikfarjam, Mehrdad

AU - Yeo, Dannel

AU - He, Hong

AU - Baldwin, Graham

AU - Fifis, Theodora

AU - Costa, Patricia

AU - Tan, Bryan

AU - Yang, Eunice

AU - Wen, Shu W.

AU - Christophi, Christopher

PY - 2013/12

Y1 - 2013/12

N2 - Background: Pancreatic adenocarcinoma has an extremely poor prognosis. The use of appropriate in vivo models is essential in devising methods to improve treatment outcomes. Methods: A pancreatic adenocarcinoma model based on tumor injection into the pancreatic head was compared with a pancreatic tail injection model in C57/BL6 mice. The murine pancreatic adenocarcinoma cell line PAN02, dispersed in MatrigelTM, was used for tumor induction. Results: Tumors developed in all animals in both models. Tumor size was more consistent within the pancreatic tail group at 20 days following induction, with no evidence of metastatic disease. Animals in the pancreatic head injection group showed signs of reduced health by 20 days following injection and developed jaundice. Microscopic liver metastases were noted in some of these animals at this time point. The overall survival of animals at 40 days following tumor induction was significantly lower in the pancreatic head injection group (0% vs. 35%; p < .001). Multiple liver metastases were noted in five of 10 (50%) animals in the head injection group, without evidence of peritoneal metastases. In the pancreatic tail injection group, 18 of 20 (90%) animals had multiple peritoneal metastases, and nine of 20 (45%) animals had evidence of isolated liver deposits. Tumors in both regions of the pancreas had similar histologic characteristics, with a dense fibrotic stroma at the interface between the tumor and the normal pancreas. Conclusion: Pancreatic head and tail orthotopic cancer models produce consistent tumors, but the patterns of tumor spread and survival differ according to the site of injection. 

AB - Background: Pancreatic adenocarcinoma has an extremely poor prognosis. The use of appropriate in vivo models is essential in devising methods to improve treatment outcomes. Methods: A pancreatic adenocarcinoma model based on tumor injection into the pancreatic head was compared with a pancreatic tail injection model in C57/BL6 mice. The murine pancreatic adenocarcinoma cell line PAN02, dispersed in MatrigelTM, was used for tumor induction. Results: Tumors developed in all animals in both models. Tumor size was more consistent within the pancreatic tail group at 20 days following induction, with no evidence of metastatic disease. Animals in the pancreatic head injection group showed signs of reduced health by 20 days following injection and developed jaundice. Microscopic liver metastases were noted in some of these animals at this time point. The overall survival of animals at 40 days following tumor induction was significantly lower in the pancreatic head injection group (0% vs. 35%; p < .001). Multiple liver metastases were noted in five of 10 (50%) animals in the head injection group, without evidence of peritoneal metastases. In the pancreatic tail injection group, 18 of 20 (90%) animals had multiple peritoneal metastases, and nine of 20 (45%) animals had evidence of isolated liver deposits. Tumors in both regions of the pancreas had similar histologic characteristics, with a dense fibrotic stroma at the interface between the tumor and the normal pancreas. Conclusion: Pancreatic head and tail orthotopic cancer models produce consistent tumors, but the patterns of tumor spread and survival differ according to the site of injection. 

KW - Animal models

KW - Metastatic neoplasm

KW - Pancreatic neoplasm

UR - http://www.scopus.com/inward/record.url?scp=84887888638&partnerID=8YFLogxK

U2 - 10.3109/08941939.2013.797057

DO - 10.3109/08941939.2013.797057

M3 - Article

VL - 26

SP - 352

EP - 359

JO - Journal of Investigative Surgery

JF - Journal of Investigative Surgery

SN - 0894-1939

IS - 6

ER -