TY - JOUR
T1 - Comparison of polypyrrole-based xanthine oxidase amperometric and potentiometric biosensors for hypoxanthine
AU - Lawal, Abdulazeez
AU - Adeloju, Samuel
PY - 2010
Y1 - 2010
N2 - The preparation and performance of xanthine oxidase electrochemical biosensor developed for the
determination of hypoxanthine is reported. Xanthine oxidase (XOD) and a mediator potassium ferrocyanide
(K4Fe(CN) were entrapped into a polypyrrole (PPy) film during galvanostatic film formation.
The optimum conditions for the formation of PPy-XOD-Fe(CN)6
4a?? film include 0.4M pyrrole, 6.2 U/mL
XOD, 40mMK4Fe(CN)6, polymerisation period of 200 s, and an applied current density of 0.5mA/cm2.
Two modes of detection, the potentiometric and amperometric modes, were investigated. The optimum
potential for the amperometric biosensing of hypoxanthine was 800mV vs. Ag/AgCl (3M KCl) in 0.05M
phosphate buffer. A comparison of the sensitivities for the amperometric and potentiometric modes
revealed that potentiometric detection was more sensitive and amperometric detection gave a wider
linear concentration range. The PPy-XOD biosensor was successfully used for the determination of fish
freshness.
AB - The preparation and performance of xanthine oxidase electrochemical biosensor developed for the
determination of hypoxanthine is reported. Xanthine oxidase (XOD) and a mediator potassium ferrocyanide
(K4Fe(CN) were entrapped into a polypyrrole (PPy) film during galvanostatic film formation.
The optimum conditions for the formation of PPy-XOD-Fe(CN)6
4a?? film include 0.4M pyrrole, 6.2 U/mL
XOD, 40mMK4Fe(CN)6, polymerisation period of 200 s, and an applied current density of 0.5mA/cm2.
Two modes of detection, the potentiometric and amperometric modes, were investigated. The optimum
potential for the amperometric biosensing of hypoxanthine was 800mV vs. Ag/AgCl (3M KCl) in 0.05M
phosphate buffer. A comparison of the sensitivities for the amperometric and potentiometric modes
revealed that potentiometric detection was more sensitive and amperometric detection gave a wider
linear concentration range. The PPy-XOD biosensor was successfully used for the determination of fish
freshness.
U2 - 10.1016/j.molcatb.2010.06.002
DO - 10.1016/j.molcatb.2010.06.002
M3 - Article
VL - 66
SP - 270
EP - 275
JO - Journal of Molecular Catalysis B: Enzymatic
JF - Journal of Molecular Catalysis B: Enzymatic
SN - 1381-1177
ER -