Comparison of immunofluorescence and immunoperoxidase for demonstration of antinuclear antibodies on HEp-2 substrate

P. G. Tipping; W. J. Dimech; G. O. Littlejohn; S. R. Holdsworth

doi:10.1093/rheumatology/26.3.188

Comparison of immunofluorescence and immunoperoxidase for demonstration of antinuclear antibodies on HEp-2 substrate

P. G. Tipping, W. J. Dimech, G. O. Littlejohn, S. R. Holdsworth

Medicine Monash Health

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

A comparison of antinuclear antibody (ANA) detection on HEp-2 cell substrate, using immunofluores-cence (IF) and immunoperoxidase (IP) techniques, was performed on 183 sera from 178 individuals, with and without connective tissue diseases. All sera were number coded and scored blindly by two independent observers. ANA was detected in 61% of sera by IF and 62% of sera by IP. Positive and negative results corresponded between the two techniques in 97%of sera. Patterns and titres corresponded in 85% of positive sera. The two independent observers scored ANA more consistently with IP than with IF. Both methods were technically simple to perform and produced consistent results with control sera. These data show that IP provides results equivalent to the traditional IF technique for demonstrating ANA on HEp-2 substrate. Fewer discordant results between two independent observers using IP suggests that this technique has technical advantages for interpretation of ANA on HEp-2 substrate.

Original language	English
Pages (from-to)	188-192
Number of pages	5
Journal	Rheumatology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1093/rheumatology/26.3.188
Publication status	Published - 1 Jun 1987

Keywords

Antinuclear antibodies
HEp-2 cell line.
Immunofluorescence
Immunoperoxidase
Rheumatoid arthritis, Scleroderma
Systemic lupus erythematosus

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10.1093/rheumatology/26.3.188

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title = "Comparison of immunofluorescence and immunoperoxidase for demonstration of antinuclear antibodies on HEp-2 substrate",

abstract = "A comparison of antinuclear antibody (ANA) detection on HEp-2 cell substrate, using immunofluores-cence (IF) and immunoperoxidase (IP) techniques, was performed on 183 sera from 178 individuals, with and without connective tissue diseases. All sera were number coded and scored blindly by two independent observers. ANA was detected in 61% of sera by IF and 62% of sera by IP. Positive and negative results corresponded between the two techniques in 97%of sera. Patterns and titres corresponded in 85% of positive sera. The two independent observers scored ANA more consistently with IP than with IF. Both methods were technically simple to perform and produced consistent results with control sera. These data show that IP provides results equivalent to the traditional IF technique for demonstrating ANA on HEp-2 substrate. Fewer discordant results between two independent observers using IP suggests that this technique has technical advantages for interpretation of ANA on HEp-2 substrate.",

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AU - Dimech, W. J.

AU - Littlejohn, G. O.

AU - Holdsworth, S. R.

PY - 1987/6/1

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N2 - A comparison of antinuclear antibody (ANA) detection on HEp-2 cell substrate, using immunofluores-cence (IF) and immunoperoxidase (IP) techniques, was performed on 183 sera from 178 individuals, with and without connective tissue diseases. All sera were number coded and scored blindly by two independent observers. ANA was detected in 61% of sera by IF and 62% of sera by IP. Positive and negative results corresponded between the two techniques in 97%of sera. Patterns and titres corresponded in 85% of positive sera. The two independent observers scored ANA more consistently with IP than with IF. Both methods were technically simple to perform and produced consistent results with control sera. These data show that IP provides results equivalent to the traditional IF technique for demonstrating ANA on HEp-2 substrate. Fewer discordant results between two independent observers using IP suggests that this technique has technical advantages for interpretation of ANA on HEp-2 substrate.

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KW - Antinuclear antibodies

KW - HEp-2 cell line.

KW - Immunofluorescence

KW - Immunoperoxidase

KW - Rheumatoid arthritis, Scleroderma

KW - Systemic lupus erythematosus

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Comparison of immunofluorescence and immunoperoxidase for demonstration of antinuclear antibodies on HEp-2 substrate

Abstract

Keywords

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