Comparison of HPLC and ATR-FTIR Methods for the Determination of Rosmarinic Acid in Aqueous Leaf Extract of Orthosiphon stamineus

Chow Jie Chen; Gabriel Akyirem Akowuah

doi:10.2174/2210315512666220429114935

Comparison of HPLC and ATR-FTIR Methods for the Determination of Rosmarinic Acid in Aqueous Leaf Extract of Orthosiphon stamineus

Chow Jie Chen, Gabriel Akyirem Akowuah

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

Background: Rosmarinic Acid (RA) is an important natural bioactive compound widely distributed in food plants. ATR-FTIR and HPLC methods for comparative determination of RA in aqueous methanolic extracts of Orthosiphon stamineus leaf samples are described.

Methods: The quantitative determination of RA was performed by using HPLC with UV detection at 340 nm and ATR-FTIR based on peak height location at 1712.29 cm^-1.

Results: The mean recovery of RA was 99.54 ± 1.23% by the HPLC method and 105.48 ± 2.76 by the ATR-IR method. The relative standard deviation for the intra-day and inter-day precision was less than 5% for the HPLC method and less than 8% for the ATR-FTIR method. The limit of detection and limit of quantification values for the HPLC method were 2 ng/ml and 10 ng/ml, respectively. The limit of detection and limit of quantification values for the ATR-FTIR method were 0.34% and 0.86% w/w, respectively. The validated methods were used for the quantification of RA in leaf samples of O. staminues. The results of HPLC and ATR-FTIR methods were comparable.

Conclusion: ATR-FTIR method is suitable for the routine determination of RA in herbal medicinal products of O. stamineus.

Original language	English
Article number	e290422204275
Number of pages	7
Journal	The Natural Products Journal
Volume	13
Issue number	1
DOIs	https://doi.org/10.2174/2210315512666220429114935
Publication status	Published - Feb 2023
Externally published	Yes

Keywords

aqueous extract
ATR-FTIR
fixed height location
HPLC
Orthosiphon stamneus
rosmarinic acid

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10.2174/2210315512666220429114935

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title = "Comparison of HPLC and ATR-FTIR Methods for the Determination of Rosmarinic Acid in Aqueous Leaf Extract of Orthosiphon stamineus",

abstract = "Background: Rosmarinic Acid (RA) is an important natural bioactive compound widely distributed in food plants. ATR-FTIR and HPLC methods for comparative determination of RA in aqueous methanolic extracts of Orthosiphon stamineus leaf samples are described. Methods: The quantitative determination of RA was performed by using HPLC with UV detection at 340 nm and ATR-FTIR based on peak height location at 1712.29 cm-1. Results: The mean recovery of RA was 99.54 ± 1.23\% by the HPLC method and 105.48 ± 2.76 by the ATR-IR method. The relative standard deviation for the intra-day and inter-day precision was less than 5\% for the HPLC method and less than 8\% for the ATR-FTIR method. The limit of detection and limit of quantification values for the HPLC method were 2 ng/ml and 10 ng/ml, respectively. The limit of detection and limit of quantification values for the ATR-FTIR method were 0.34\% and 0.86\% w/w, respectively. The validated methods were used for the quantification of RA in leaf samples of O. staminues. The results of HPLC and ATR-FTIR methods were comparable. Conclusion: ATR-FTIR method is suitable for the routine determination of RA in herbal medicinal products of O. stamineus.",

keywords = "aqueous extract, ATR-FTIR, fixed height location, HPLC, Orthosiphon stamneus, rosmarinic acid",

author = "Chen, \{Chow Jie\} and Akowuah, \{Gabriel Akyirem\}",

note = "Funding Information: The research work was financially supported by the UC-SI University Research Grant Scheme (Proj-In-FPS-025), awarded by the Centre of Excellence for Research, Value Innovation and Entrepreneurship (CERVIE), UCSI University Publisher Copyright: {\textcopyright} 2023 Bentham Science Publishers.",

year = "2023",

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doi = "10.2174/2210315512666220429114935",

language = "English",

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T1 - Comparison of HPLC and ATR-FTIR Methods for the Determination of Rosmarinic Acid in Aqueous Leaf Extract of Orthosiphon stamineus

AU - Chen, Chow Jie

AU - Akowuah, Gabriel Akyirem

N1 - Funding Information: The research work was financially supported by the UC-SI University Research Grant Scheme (Proj-In-FPS-025), awarded by the Centre of Excellence for Research, Value Innovation and Entrepreneurship (CERVIE), UCSI University Publisher Copyright: © 2023 Bentham Science Publishers.

PY - 2023/2

Y1 - 2023/2

N2 - Background: Rosmarinic Acid (RA) is an important natural bioactive compound widely distributed in food plants. ATR-FTIR and HPLC methods for comparative determination of RA in aqueous methanolic extracts of Orthosiphon stamineus leaf samples are described. Methods: The quantitative determination of RA was performed by using HPLC with UV detection at 340 nm and ATR-FTIR based on peak height location at 1712.29 cm-1. Results: The mean recovery of RA was 99.54 ± 1.23% by the HPLC method and 105.48 ± 2.76 by the ATR-IR method. The relative standard deviation for the intra-day and inter-day precision was less than 5% for the HPLC method and less than 8% for the ATR-FTIR method. The limit of detection and limit of quantification values for the HPLC method were 2 ng/ml and 10 ng/ml, respectively. The limit of detection and limit of quantification values for the ATR-FTIR method were 0.34% and 0.86% w/w, respectively. The validated methods were used for the quantification of RA in leaf samples of O. staminues. The results of HPLC and ATR-FTIR methods were comparable. Conclusion: ATR-FTIR method is suitable for the routine determination of RA in herbal medicinal products of O. stamineus.

AB - Background: Rosmarinic Acid (RA) is an important natural bioactive compound widely distributed in food plants. ATR-FTIR and HPLC methods for comparative determination of RA in aqueous methanolic extracts of Orthosiphon stamineus leaf samples are described. Methods: The quantitative determination of RA was performed by using HPLC with UV detection at 340 nm and ATR-FTIR based on peak height location at 1712.29 cm-1. Results: The mean recovery of RA was 99.54 ± 1.23% by the HPLC method and 105.48 ± 2.76 by the ATR-IR method. The relative standard deviation for the intra-day and inter-day precision was less than 5% for the HPLC method and less than 8% for the ATR-FTIR method. The limit of detection and limit of quantification values for the HPLC method were 2 ng/ml and 10 ng/ml, respectively. The limit of detection and limit of quantification values for the ATR-FTIR method were 0.34% and 0.86% w/w, respectively. The validated methods were used for the quantification of RA in leaf samples of O. staminues. The results of HPLC and ATR-FTIR methods were comparable. Conclusion: ATR-FTIR method is suitable for the routine determination of RA in herbal medicinal products of O. stamineus.

KW - aqueous extract

KW - ATR-FTIR

KW - fixed height location

KW - HPLC

KW - Orthosiphon stamneus

KW - rosmarinic acid

UR - https://www.scopus.com/pages/publications/85144145304

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DO - 10.2174/2210315512666220429114935

M3 - Article

AN - SCOPUS:85144145304

SN - 2210-3155

VL - 13

JO - The Natural Products Journal

JF - The Natural Products Journal

IS - 1

M1 - e290422204275

ER -

Comparison of HPLC and ATR-FTIR Methods for the Determination of Rosmarinic Acid in Aqueous Leaf Extract of Orthosiphon stamineus

Abstract

Keywords

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