Comparative study of electron mediators used in the electrochemical oxidation of NADH

Beatriu Prieto-Simón; Esteve Fàbregas

doi:10.1016/j.bios.2003.10.010

Comparative study of electron mediators used in the electrochemical oxidation of NADH

Beatriu Prieto-Simón
, Esteve Fàbregas

Research output: Contribution to journal › Article › Research › peer-review

93 Citations (Scopus)

Abstract

A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared.

Original language	English
Pages (from-to)	1131-1138
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	19
Issue number	10
DOIs	https://doi.org/10.1016/j.bios.2003.10.010
Publication status	Published - 15 May 2004
Externally published	Yes

Keywords

Amperometric detection of NADH
Chemically modified electrodes
Redox mediators

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10.1016/j.bios.2003.10.010

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AU - Prieto-Simón, Beatriu

AU - Fàbregas, Esteve

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N2 - A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared.

AB - A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (II), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared.

KW - Amperometric detection of NADH

KW - Chemically modified electrodes

KW - Redox mediators

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JO - Biosensors and Bioelectronics

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IS - 10

ER -

Comparative study of electron mediators used in the electrochemical oxidation of NADH

Abstract

Keywords

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