TY - JOUR
T1 - Comparative Proteomic Analysis of the Venoms from the Most Dangerous Scorpions in Morocco
T2 - Androctonus mauritanicus and Buthus occitanus
AU - Hilal, Ines
AU - Khourcha, Soukaina
AU - Safi, Amal
AU - Hmyene, Abdelaziz
AU - Asnawi, Syafiq
AU - Othman, Iekhsan
AU - Stöcklin, Reto
AU - Oukkache, Naoual
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/5/5
Y1 - 2023/5/5
N2 - Morocco is known to harbor two of the world’s most dangerous scorpion species: the black Androctonus mauritanicus (Am) and the yellow Buthus occitanus (Bo), responsible for 83% and 14% of severe envenomation cases, respectively. Scorpion venom is a mixture of biological molecules of variable structures and activities, most of which are proteins of low molecular weights referred to as toxins. In addition to toxins, scorpion venoms also contain biogenic amines, polyamines, and enzymes. With the aim of investigating the composition of the Am and Bo venoms, we conducted an analysis of the venoms by mass spectrometry (ESI-MS) after separation by reversed-phase HPLC chromatography. Results from a total of 19 fractions obtained for the Am venom versus 22 fractions for the Bo venom allowed the identification of approximately 410 and 252 molecular masses, respectively. In both venoms, the most abundant toxins were found to range between 2–5 kDa and 6–8 kDa. This proteomic analysis not only allowed the drawing of an extensive mass fingerprint of the Androctonus mauritanicus and Buthus occitanus venoms but also provided a better insight into the nature of their toxins.
AB - Morocco is known to harbor two of the world’s most dangerous scorpion species: the black Androctonus mauritanicus (Am) and the yellow Buthus occitanus (Bo), responsible for 83% and 14% of severe envenomation cases, respectively. Scorpion venom is a mixture of biological molecules of variable structures and activities, most of which are proteins of low molecular weights referred to as toxins. In addition to toxins, scorpion venoms also contain biogenic amines, polyamines, and enzymes. With the aim of investigating the composition of the Am and Bo venoms, we conducted an analysis of the venoms by mass spectrometry (ESI-MS) after separation by reversed-phase HPLC chromatography. Results from a total of 19 fractions obtained for the Am venom versus 22 fractions for the Bo venom allowed the identification of approximately 410 and 252 molecular masses, respectively. In both venoms, the most abundant toxins were found to range between 2–5 kDa and 6–8 kDa. This proteomic analysis not only allowed the drawing of an extensive mass fingerprint of the Androctonus mauritanicus and Buthus occitanus venoms but also provided a better insight into the nature of their toxins.
KW - Androctonus mauritanicus
KW - Buthus occitanus
KW - mass fingerprinting
KW - mass spectrometry
KW - proteomics
KW - scorpion
KW - venom
UR - http://www.scopus.com/inward/record.url?scp=85160250717&partnerID=8YFLogxK
U2 - 10.3390/life13051133
DO - 10.3390/life13051133
M3 - Article
C2 - 37240778
AN - SCOPUS:85160250717
SN - 2075-1729
VL - 13
JO - Life
JF - Life
IS - 5
M1 - 1133
ER -