Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea

Claire E. Ryan, Cassey S. Simbiken, Paul A. Agius, Joyce Allen, Joyce Sauk, Petronia Kaima, Zure Kombati, Peter Siba, John M. Kaldor, Andrew Vallely, Male Circumcision Acceptability Team, Impact Study MCAIS Team

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2 Citations (Scopus)

Abstract

Background Infection with herpes simplex virus type 2 (HSV-2) is common worldwide and an important risk factor for HIV infection. Aetiological diagnosis of HSV-2 is typically determined with the use of commercially available type-specific enzyme-linked immunosorbent assays (ELISAs). This study aimed to determine the prevalence of HSV-2 among people attending sexual health clinics in the Highlands of Papua New Guinea. The study also aimed to compare the performance of two type-specific ELISA assays, the Kalon and HerpeSelect glycoprotein G2 assays, in this context. Methods: Participants were recruited as part of a longitudinal sexual health study. Participants attended four appointments over a 12-month period and had blood taken for HSV-2 serology at each time point. Both the Kalon and HerpeSelect assays were performed as per manufacturer's instructions. Results: A total of 132 participants were tested for HSV-2 using the Kalon and HerpeSelect ELISAs. HSV-2 prevalence was 52% (95% CI, 43-60) and 61% (95% CI, 52-69) with Kalon and HerpeSelect assays respectively. There was high concordance (87%, ?≤0.75, P<0.001, n≤115) between the two assays at the manufacturer recommended index value cut-offs. For participants with discordant results at baseline, (n≤16), three sero-conversions were observed over the 12-month period when sequential sera was tested. Conclusions: A high HSV-2 prevalence was observed in this clinic-based population. Our longitudinal data indicate the higher prevalence of HSV-2 detected with the HerpeSelect ELISA was likely due to false positives rather than a higher sensitivity in the early stages of infection.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalSexual Health
Volume11
Issue number6
DOIs
Publication statusPublished - 1 Dec 2014
Externally publishedYes

Cite this

Ryan, Claire E. ; Simbiken, Cassey S. ; Agius, Paul A. ; Allen, Joyce ; Sauk, Joyce ; Kaima, Petronia ; Kombati, Zure ; Siba, Peter ; Kaldor, John M. ; Vallely, Andrew ; Male Circumcision Acceptability Team ; Impact Study MCAIS Team. / Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea. In: Sexual Health. 2014 ; Vol. 11, No. 6. pp. 575-579.
@article{088ad9c64d2c42148d35ebbb8c49a445,
title = "Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea",
abstract = "Background Infection with herpes simplex virus type 2 (HSV-2) is common worldwide and an important risk factor for HIV infection. Aetiological diagnosis of HSV-2 is typically determined with the use of commercially available type-specific enzyme-linked immunosorbent assays (ELISAs). This study aimed to determine the prevalence of HSV-2 among people attending sexual health clinics in the Highlands of Papua New Guinea. The study also aimed to compare the performance of two type-specific ELISA assays, the Kalon and HerpeSelect glycoprotein G2 assays, in this context. Methods: Participants were recruited as part of a longitudinal sexual health study. Participants attended four appointments over a 12-month period and had blood taken for HSV-2 serology at each time point. Both the Kalon and HerpeSelect assays were performed as per manufacturer's instructions. Results: A total of 132 participants were tested for HSV-2 using the Kalon and HerpeSelect ELISAs. HSV-2 prevalence was 52{\%} (95{\%} CI, 43-60) and 61{\%} (95{\%} CI, 52-69) with Kalon and HerpeSelect assays respectively. There was high concordance (87{\%}, ?≤0.75, P<0.001, n≤115) between the two assays at the manufacturer recommended index value cut-offs. For participants with discordant results at baseline, (n≤16), three sero-conversions were observed over the 12-month period when sequential sera was tested. Conclusions: A high HSV-2 prevalence was observed in this clinic-based population. Our longitudinal data indicate the higher prevalence of HSV-2 detected with the HerpeSelect ELISA was likely due to false positives rather than a higher sensitivity in the early stages of infection.",
author = "Ryan, {Claire E.} and Simbiken, {Cassey S.} and Agius, {Paul A.} and Joyce Allen and Joyce Sauk and Petronia Kaima and Zure Kombati and Peter Siba and Kaldor, {John M.} and Andrew Vallely and {Male Circumcision Acceptability Team} and {Impact Study MCAIS Team}",
year = "2014",
month = "12",
day = "1",
doi = "10.1071/SH14055",
language = "English",
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Ryan, CE, Simbiken, CS, Agius, PA, Allen, J, Sauk, J, Kaima, P, Kombati, Z, Siba, P, Kaldor, JM, Vallely, A, Male Circumcision Acceptability Team & Impact Study MCAIS Team 2014, 'Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea', Sexual Health, vol. 11, no. 6, pp. 575-579. https://doi.org/10.1071/SH14055

Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea. / Ryan, Claire E.; Simbiken, Cassey S.; Agius, Paul A.; Allen, Joyce; Sauk, Joyce; Kaima, Petronia; Kombati, Zure; Siba, Peter; Kaldor, John M.; Vallely, Andrew; Male Circumcision Acceptability Team; Impact Study MCAIS Team.

In: Sexual Health, Vol. 11, No. 6, 01.12.2014, p. 575-579.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparative performance of the Kalon and HerpeSelect enzyme-linked immunosorbant assays to determine the prevalence of herpes simplex virus type 2 in Papua New Guinea

AU - Ryan, Claire E.

AU - Simbiken, Cassey S.

AU - Agius, Paul A.

AU - Allen, Joyce

AU - Sauk, Joyce

AU - Kaima, Petronia

AU - Kombati, Zure

AU - Siba, Peter

AU - Kaldor, John M.

AU - Vallely, Andrew

AU - Male Circumcision Acceptability Team

AU - Impact Study MCAIS Team

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Background Infection with herpes simplex virus type 2 (HSV-2) is common worldwide and an important risk factor for HIV infection. Aetiological diagnosis of HSV-2 is typically determined with the use of commercially available type-specific enzyme-linked immunosorbent assays (ELISAs). This study aimed to determine the prevalence of HSV-2 among people attending sexual health clinics in the Highlands of Papua New Guinea. The study also aimed to compare the performance of two type-specific ELISA assays, the Kalon and HerpeSelect glycoprotein G2 assays, in this context. Methods: Participants were recruited as part of a longitudinal sexual health study. Participants attended four appointments over a 12-month period and had blood taken for HSV-2 serology at each time point. Both the Kalon and HerpeSelect assays were performed as per manufacturer's instructions. Results: A total of 132 participants were tested for HSV-2 using the Kalon and HerpeSelect ELISAs. HSV-2 prevalence was 52% (95% CI, 43-60) and 61% (95% CI, 52-69) with Kalon and HerpeSelect assays respectively. There was high concordance (87%, ?≤0.75, P<0.001, n≤115) between the two assays at the manufacturer recommended index value cut-offs. For participants with discordant results at baseline, (n≤16), three sero-conversions were observed over the 12-month period when sequential sera was tested. Conclusions: A high HSV-2 prevalence was observed in this clinic-based population. Our longitudinal data indicate the higher prevalence of HSV-2 detected with the HerpeSelect ELISA was likely due to false positives rather than a higher sensitivity in the early stages of infection.

AB - Background Infection with herpes simplex virus type 2 (HSV-2) is common worldwide and an important risk factor for HIV infection. Aetiological diagnosis of HSV-2 is typically determined with the use of commercially available type-specific enzyme-linked immunosorbent assays (ELISAs). This study aimed to determine the prevalence of HSV-2 among people attending sexual health clinics in the Highlands of Papua New Guinea. The study also aimed to compare the performance of two type-specific ELISA assays, the Kalon and HerpeSelect glycoprotein G2 assays, in this context. Methods: Participants were recruited as part of a longitudinal sexual health study. Participants attended four appointments over a 12-month period and had blood taken for HSV-2 serology at each time point. Both the Kalon and HerpeSelect assays were performed as per manufacturer's instructions. Results: A total of 132 participants were tested for HSV-2 using the Kalon and HerpeSelect ELISAs. HSV-2 prevalence was 52% (95% CI, 43-60) and 61% (95% CI, 52-69) with Kalon and HerpeSelect assays respectively. There was high concordance (87%, ?≤0.75, P<0.001, n≤115) between the two assays at the manufacturer recommended index value cut-offs. For participants with discordant results at baseline, (n≤16), three sero-conversions were observed over the 12-month period when sequential sera was tested. Conclusions: A high HSV-2 prevalence was observed in this clinic-based population. Our longitudinal data indicate the higher prevalence of HSV-2 detected with the HerpeSelect ELISA was likely due to false positives rather than a higher sensitivity in the early stages of infection.

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U2 - 10.1071/SH14055

DO - 10.1071/SH14055

M3 - Article

VL - 11

SP - 575

EP - 579

JO - Sexual Health

JF - Sexual Health

SN - 1448-5028

IS - 6

ER -