Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia

Andrew H. Buultjens, Koen Vandelannoote, Conor J. Meehan, Miriam Eddyani, Bouke C. de Jong, Janet A. M. Fyfe, Maria Globan, Nicholas J. Tobias, Jessica L. Porter, Takehiro Tomita, Ee Laine Tay, Torsten Seemann, Benjamin P. Howden, Paul D. R. Johnson, Timothy P. Stinear

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Since 2000, cases of the neglected tropical disease Buruli ulcer, caused by infection with Mycobacterium ulcerans, have increased 100-fold around Melbourne (population 4.4 million), the capital of Victoria, in temperate southeastern Australia. The reasons for this increase are unclear. Here, we used whole-genome sequence comparisons of 178 M. ulcerans isolates obtained primarily from human clinical specimens, spanning 70 years, to model the population dynamics of this pathogen from this region. Using phylogeographic and advanced Bayesian phylogenetic approaches, we found that there has been a migration of the pathogen from the east end of the state, beginning in the 1980s, 300 km west to the major human population center around Melbourne. This move was then followed by a significant increase in M. ulcerans population size. These analyses inform our thinking around Buruli ulcer transmission and control, indicating that M. ulcerans is introduced to a new environment and then expands, rather than it being from the awakening of a quiescent pathogen reservoir.

Original languageEnglish
Article numbere02612-17
Number of pages15
JournalApplied and Environmental Microbiology
Volume84
Issue number8
DOIs
Publication statusPublished - 1 Apr 2018
Externally publishedYes

Keywords

  • Australia
  • Buruli ulcer
  • Genomics
  • Mycobacterium ulcerans
  • Phylogenetics
  • Population genetics
  • Public health

Cite this

Buultjens, A. H., Vandelannoote, K., Meehan, C. J., Eddyani, M., de Jong, B. C., Fyfe, J. A. M., ... Stinear, T. P. (2018). Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia. Applied and Environmental Microbiology, 84(8), [e02612-17]. https://doi.org/10.1128/AEM.02612-17
Buultjens, Andrew H. ; Vandelannoote, Koen ; Meehan, Conor J. ; Eddyani, Miriam ; de Jong, Bouke C. ; Fyfe, Janet A. M. ; Globan, Maria ; Tobias, Nicholas J. ; Porter, Jessica L. ; Tomita, Takehiro ; Tay, Ee Laine ; Seemann, Torsten ; Howden, Benjamin P. ; Johnson, Paul D. R. ; Stinear, Timothy P. / Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia. In: Applied and Environmental Microbiology. 2018 ; Vol. 84, No. 8.
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title = "Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia",
abstract = "Since 2000, cases of the neglected tropical disease Buruli ulcer, caused by infection with Mycobacterium ulcerans, have increased 100-fold around Melbourne (population 4.4 million), the capital of Victoria, in temperate southeastern Australia. The reasons for this increase are unclear. Here, we used whole-genome sequence comparisons of 178 M. ulcerans isolates obtained primarily from human clinical specimens, spanning 70 years, to model the population dynamics of this pathogen from this region. Using phylogeographic and advanced Bayesian phylogenetic approaches, we found that there has been a migration of the pathogen from the east end of the state, beginning in the 1980s, 300 km west to the major human population center around Melbourne. This move was then followed by a significant increase in M. ulcerans population size. These analyses inform our thinking around Buruli ulcer transmission and control, indicating that M. ulcerans is introduced to a new environment and then expands, rather than it being from the awakening of a quiescent pathogen reservoir.",
keywords = "Australia, Buruli ulcer, Genomics, Mycobacterium ulcerans, Phylogenetics, Population genetics, Public health",
author = "Buultjens, {Andrew H.} and Koen Vandelannoote and Meehan, {Conor J.} and Miriam Eddyani and {de Jong}, {Bouke C.} and Fyfe, {Janet A. M.} and Maria Globan and Tobias, {Nicholas J.} and Porter, {Jessica L.} and Takehiro Tomita and Tay, {Ee Laine} and Torsten Seemann and Howden, {Benjamin P.} and Johnson, {Paul D. R.} and Stinear, {Timothy P.}",
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Buultjens, AH, Vandelannoote, K, Meehan, CJ, Eddyani, M, de Jong, BC, Fyfe, JAM, Globan, M, Tobias, NJ, Porter, JL, Tomita, T, Tay, EL, Seemann, T, Howden, BP, Johnson, PDR & Stinear, TP 2018, 'Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia' Applied and Environmental Microbiology, vol. 84, no. 8, e02612-17. https://doi.org/10.1128/AEM.02612-17

Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia. / Buultjens, Andrew H.; Vandelannoote, Koen; Meehan, Conor J.; Eddyani, Miriam; de Jong, Bouke C.; Fyfe, Janet A. M.; Globan, Maria; Tobias, Nicholas J.; Porter, Jessica L.; Tomita, Takehiro; Tay, Ee Laine; Seemann, Torsten; Howden, Benjamin P.; Johnson, Paul D. R.; Stinear, Timothy P.

In: Applied and Environmental Microbiology, Vol. 84, No. 8, e02612-17, 01.04.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparative genomics shows that Mycobacterium ulcerans migration and expansion preceded the rise of Buruli ulcer in southeastern Australia

AU - Buultjens, Andrew H.

AU - Vandelannoote, Koen

AU - Meehan, Conor J.

AU - Eddyani, Miriam

AU - de Jong, Bouke C.

AU - Fyfe, Janet A. M.

AU - Globan, Maria

AU - Tobias, Nicholas J.

AU - Porter, Jessica L.

AU - Tomita, Takehiro

AU - Tay, Ee Laine

AU - Seemann, Torsten

AU - Howden, Benjamin P.

AU - Johnson, Paul D. R.

AU - Stinear, Timothy P.

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Since 2000, cases of the neglected tropical disease Buruli ulcer, caused by infection with Mycobacterium ulcerans, have increased 100-fold around Melbourne (population 4.4 million), the capital of Victoria, in temperate southeastern Australia. The reasons for this increase are unclear. Here, we used whole-genome sequence comparisons of 178 M. ulcerans isolates obtained primarily from human clinical specimens, spanning 70 years, to model the population dynamics of this pathogen from this region. Using phylogeographic and advanced Bayesian phylogenetic approaches, we found that there has been a migration of the pathogen from the east end of the state, beginning in the 1980s, 300 km west to the major human population center around Melbourne. This move was then followed by a significant increase in M. ulcerans population size. These analyses inform our thinking around Buruli ulcer transmission and control, indicating that M. ulcerans is introduced to a new environment and then expands, rather than it being from the awakening of a quiescent pathogen reservoir.

AB - Since 2000, cases of the neglected tropical disease Buruli ulcer, caused by infection with Mycobacterium ulcerans, have increased 100-fold around Melbourne (population 4.4 million), the capital of Victoria, in temperate southeastern Australia. The reasons for this increase are unclear. Here, we used whole-genome sequence comparisons of 178 M. ulcerans isolates obtained primarily from human clinical specimens, spanning 70 years, to model the population dynamics of this pathogen from this region. Using phylogeographic and advanced Bayesian phylogenetic approaches, we found that there has been a migration of the pathogen from the east end of the state, beginning in the 1980s, 300 km west to the major human population center around Melbourne. This move was then followed by a significant increase in M. ulcerans population size. These analyses inform our thinking around Buruli ulcer transmission and control, indicating that M. ulcerans is introduced to a new environment and then expands, rather than it being from the awakening of a quiescent pathogen reservoir.

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KW - Phylogenetics

KW - Population genetics

KW - Public health

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U2 - 10.1128/AEM.02612-17

DO - 10.1128/AEM.02612-17

M3 - Article

VL - 84

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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