Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways

Nicholas R. Thomson, Debra J. Clayton, Daniel Windhorst, Georgios Vernikos, Susanne Davidson, Carol Churcher, Michael A. Quail, Mark Stevens, Michael A. Jones, Michael Watson, Andy Barron, Abigail Layton, Derek Pickard, Robert A. Kingsley, Alex Bignell, Louise Clark, Barbara Harris, Doug Ormond, Zahra Abdellah, Karen BrooksInna Cherevach, Tracey Chillingworth, John Woodward, Halina Norberczak, Angela Lord, Claire Arrowsmith, Kay Jagels, Sharon Moule, Karen Mungall, Mandy Sanders, Sally Whitehead, Jose A. Chabalgoity, Duncan Maskell, Tom Humphrey, Mark Roberts, Paul A. Barrow, Gordon Dougan, Julian Parkhill

Research output: Contribution to journalArticleResearchpeer-review

294 Citations (Scopus)

Abstract

We have determined the complete genome sequences of a host-promiscuous Salmonella enterica serovar Enteritidis PT4 isolate P125109 and a chicken-restricted Salmonella enterica serovar Gallinarum isolate 287/91. Genome comparisons between these and other Salmonella isolates indicate that S. Gallinarum 287/91 is a recently evolved descendent of S. Enteritidis. Significantly, the genome of S. Gallinarum has undergone extensive degradation through deletion and pseudogene formation. Comparison of the pseudogenes in S. Gallinarum with those identified previously in other host-adapted bacteria reveals the loss of many common functional traits and provides insights into possible mechanisms of host and tissue adaptation. We propose that experimental analysis in chickens and mice of S. Enteritidis-harboring mutations in functional homologs of the pseudogenes present in S. Gallinarum could provide an experimentally tractable route toward unraveling the genetic basis of host adaptation in S. enterica.

Original languageEnglish
Pages (from-to)1624-1637
Number of pages14
JournalGenome Research
Volume18
Issue number10
DOIs
Publication statusPublished - 1 Oct 2008
Externally publishedYes

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