Abstract
The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.
| Original language | English |
|---|---|
| Pages (from-to) | 1037-1041 |
| Number of pages | 5 |
| Journal | Canadian Journal of Physiology and Pharmacology |
| Volume | 73 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jan 1995 |
| Externally published | Yes |
Keywords
- amylin
- calcitonin
- calcitonin gene related peptide
- monkey
- rat
- receptor
Cite this
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Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain. / Christopoulos, G.; Paxinos, G.; Huang, X. F.; Beaumont, K.; Toga, A. W.; Sexton, P. M.
In: Canadian Journal of Physiology and Pharmacology, Vol. 73, No. 7, 01.01.1995, p. 1037-1041.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain
AU - Christopoulos, G.
AU - Paxinos, G.
AU - Huang, X. F.
AU - Beaumont, K.
AU - Toga, A. W.
AU - Sexton, P. M.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.
AB - The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.
KW - amylin
KW - calcitonin
KW - calcitonin gene related peptide
KW - monkey
KW - rat
KW - receptor
UR - http://www.scopus.com/inward/record.url?scp=0028868766&partnerID=8YFLogxK
U2 - 10.1139/y95-146
DO - 10.1139/y95-146
M3 - Article
C2 - 8846397
AN - SCOPUS:0028868766
VL - 73
SP - 1037
EP - 1041
JO - Canadian Journal of Physiology and Pharmacology
JF - Canadian Journal of Physiology and Pharmacology
SN - 0008-4212
IS - 7
ER -