Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain

G. Christopoulos, G. Paxinos, X. F. Huang, K. Beaumont, A. W. Toga, P. M. Sexton

Research output: Contribution to journalArticleResearchpeer-review

66 Citations (Scopus)

Abstract

The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.

Original languageEnglish
Pages (from-to)1037-1041
Number of pages5
JournalCanadian Journal of Physiology and Pharmacology
Volume73
Issue number7
DOIs
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Keywords

  • amylin
  • calcitonin
  • calcitonin gene related peptide
  • monkey
  • rat
  • receptor

Cite this

@article{2a2ea32eb7e641b4be60bdb900b18294,
title = "Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain",
abstract = "The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.",
keywords = "amylin, calcitonin, calcitonin gene related peptide, monkey, rat, receptor",
author = "G. Christopoulos and G. Paxinos and Huang, {X. F.} and K. Beaumont and Toga, {A. W.} and Sexton, {P. M.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1139/y95-146",
language = "English",
volume = "73",
pages = "1037--1041",
journal = "Canadian Journal of Physiology and Pharmacology",
issn = "0008-4212",
publisher = "NRC Research Press",
number = "7",

}

Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain. / Christopoulos, G.; Paxinos, G.; Huang, X. F.; Beaumont, K.; Toga, A. W.; Sexton, P. M.

In: Canadian Journal of Physiology and Pharmacology, Vol. 73, No. 7, 01.01.1995, p. 1037-1041.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparative distribution of receptors for amylin and the related peptides calcitonin gene related peptide and calcitonin in rat and monkey brain

AU - Christopoulos, G.

AU - Paxinos, G.

AU - Huang, X. F.

AU - Beaumont, K.

AU - Toga, A. W.

AU - Sexton, P. M.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.

AB - The distribution of amylin receptors ( 125 I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) ( 125 I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125 I-labelled rat CGRPα; monkey, 125 I-labelled human CGRPα). In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125 I-labelled salmon CT and 125 I-labelled rat CGRPα. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125 I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRPα having relatively lower affinity for the primate amylin receptors.

KW - amylin

KW - calcitonin

KW - calcitonin gene related peptide

KW - monkey

KW - rat

KW - receptor

UR - http://www.scopus.com/inward/record.url?scp=0028868766&partnerID=8YFLogxK

U2 - 10.1139/y95-146

DO - 10.1139/y95-146

M3 - Article

C2 - 8846397

AN - SCOPUS:0028868766

VL - 73

SP - 1037

EP - 1041

JO - Canadian Journal of Physiology and Pharmacology

JF - Canadian Journal of Physiology and Pharmacology

SN - 0008-4212

IS - 7

ER -