TY - JOUR
T1 - Comparative antiproliferative and receptor binding activities of interferons α and β on lymphoblastoid and melanoma cells
AU - Hertzog, P. J.
AU - Johns, T. G.
AU - Callister, K. A.
AU - Dinatale, A.
AU - Linnane, A. W.
PY - 1990/12/1
Y1 - 1990/12/1
N2 - The relative antiproliferative and receptor binding characteristics of the hitherto little-characterized interferon α4a on cells of lymphoid and epithelial origin are compared with two other type I interferons, α2a and β. Using the lymphoblastoid cell line, Daudi, interferons α4a and α2b had similar antiproliferative activity, and were about 10-fold more active than IFNβ. By contrast, using the melanoma cell line Sk-Mel-28, IFNβ was the most active, whereas IFNα2b and IFNα4a were respectively 60-fold and > 1000-fold less active than on Daudi cells. Receptor binding did not correlate with antiproliferative sensitivities, but confirmed a shared receptor component for these three interferons. These results indicate that the antiproliferative activities of three type I IFNs differs markedly on different cell types and that this is unlikely to be due to receptor binding, but more likely a post receptor binding event.
AB - The relative antiproliferative and receptor binding characteristics of the hitherto little-characterized interferon α4a on cells of lymphoid and epithelial origin are compared with two other type I interferons, α2a and β. Using the lymphoblastoid cell line, Daudi, interferons α4a and α2b had similar antiproliferative activity, and were about 10-fold more active than IFNβ. By contrast, using the melanoma cell line Sk-Mel-28, IFNβ was the most active, whereas IFNα2b and IFNα4a were respectively 60-fold and > 1000-fold less active than on Daudi cells. Receptor binding did not correlate with antiproliferative sensitivities, but confirmed a shared receptor component for these three interferons. These results indicate that the antiproliferative activities of three type I IFNs differs markedly on different cell types and that this is unlikely to be due to receptor binding, but more likely a post receptor binding event.
UR - http://www.scopus.com/inward/record.url?scp=0025611087&partnerID=8YFLogxK
M3 - Article
C2 - 2151021
AN - SCOPUS:0025611087
VL - 22
SP - 1095
EP - 1102
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
SN - 1039-9712
IS - 6
ER -