TY - JOUR
T1 - Comparative analysis on metal removal potential of exopolymeric substances with live and dead cells of bacteria
AU - Cheah, Caleb
AU - Cheow, Yuen Lin
AU - Ting, Adeline Su Yien
N1 - Funding Information:
The authors are grateful to the Malaysian Ministry of Education (MOE) for the funding under the FRGS grant scheme (FRGS/1/2018/STG03/MUSM/02/1). The authors also thank Monash University Malaysia for providing the resources and facilities to conduct the project.
Funding Information:
The authors are grateful to the Malaysian Ministry of Education (MOE) for the funding under the FRGS grant scheme (FRGS/1/2018/STG03/MUSM/02/1). The authors also thank Monash University Malaysia for providing the resources and facilities to conduct the project.
Funding Information:
This project was funded by the Malaysian Ministry of Education (MOE) under the FRGS Grant scheme (FRGS/1/2018/STG03/MUSM/02/1).
Publisher Copyright:
© 2022, University of Tehran.
PY - 2022/2
Y1 - 2022/2
N2 - This study investigated and compared the metal removal efficacy of exopolymeric substances (EPS), live and dead cells of Bacillus cereus, Staphylococcus aureus, Stenotrophomonas maltophilia and Pseudomonas fluorescens. Results revealed that dead cells of Gram-positive bacteria (B. cereus, S. aureus) showed better metal removal potential compared to live cells, while the contrary was observed for Gram-negative bacteria (S. maltophilia, P. fluorescens). The EPS from all bacterial isolates demonstrated the least efficacy in metal removal potential (9–30%) compared to live (12–63%) and dead (16–70%) cells. Comparing among bacterial isolates, the EPS of B. cereus was the most effective in biosorbing all metals (Cu: 27.94%, Pb: 34.29%, Zn: 23.15%, Cd: 14.65%, Cr: 26.15%). These were, however, lower than the amount of metals removed by live (Cu: 44.82%, Pb: 53.64%, Zn: 39.13%, Cd: 19.85%, Cr: 38.75%) and dead (Cu: 63.26%, Pb: 70.16%, Zn: 58.86%, Cd: 57.68%, Cr: 56.48%) cells of B. cereus. Fourier-transform infrared spectroscopy (FTIR) analysis suggests that the hydroxyl groups in EPS were primarily involved in metal biosorption. Mean metal removal in multi-metal systems (31.75 ± 13.21%) were significantly inferior compared to single-metal systems (36.21 ± 13.24%). This study revealed that EPS has the potential for metal removal although the amount of metal removed is lower than levels removed by live and dead cells. EPS remains an interesting alternative as the use of this non-toxic biomolecule is safer compared to live and dead cells. EPS can be further improved upon via cultivation methods or pre-treatment, to establish their feasible use for wastewater treatment.
AB - This study investigated and compared the metal removal efficacy of exopolymeric substances (EPS), live and dead cells of Bacillus cereus, Staphylococcus aureus, Stenotrophomonas maltophilia and Pseudomonas fluorescens. Results revealed that dead cells of Gram-positive bacteria (B. cereus, S. aureus) showed better metal removal potential compared to live cells, while the contrary was observed for Gram-negative bacteria (S. maltophilia, P. fluorescens). The EPS from all bacterial isolates demonstrated the least efficacy in metal removal potential (9–30%) compared to live (12–63%) and dead (16–70%) cells. Comparing among bacterial isolates, the EPS of B. cereus was the most effective in biosorbing all metals (Cu: 27.94%, Pb: 34.29%, Zn: 23.15%, Cd: 14.65%, Cr: 26.15%). These were, however, lower than the amount of metals removed by live (Cu: 44.82%, Pb: 53.64%, Zn: 39.13%, Cd: 19.85%, Cr: 38.75%) and dead (Cu: 63.26%, Pb: 70.16%, Zn: 58.86%, Cd: 57.68%, Cr: 56.48%) cells of B. cereus. Fourier-transform infrared spectroscopy (FTIR) analysis suggests that the hydroxyl groups in EPS were primarily involved in metal biosorption. Mean metal removal in multi-metal systems (31.75 ± 13.21%) were significantly inferior compared to single-metal systems (36.21 ± 13.24%). This study revealed that EPS has the potential for metal removal although the amount of metal removed is lower than levels removed by live and dead cells. EPS remains an interesting alternative as the use of this non-toxic biomolecule is safer compared to live and dead cells. EPS can be further improved upon via cultivation methods or pre-treatment, to establish their feasible use for wastewater treatment.
KW - Bacteria
KW - Biosorption
KW - Exopolymeric substances
KW - Multi-metal solutions
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=85122740077&partnerID=8YFLogxK
U2 - 10.1007/s41742-021-00386-2
DO - 10.1007/s41742-021-00386-2
M3 - Article
AN - SCOPUS:85122740077
SN - 1735-6865
VL - 16
JO - International Journal of Environmental Research
JF - International Journal of Environmental Research
IS - 1
M1 - 12
ER -