Comparative analysis of the first complete Enterococcus faecium genome

Margaret MC Lam, Torsten Seemann, Dieter Mark Bulach, Simon Leigh Gladman, Honglei Chen, Volker Haring, Robert Moore, Susan Alicia Ballard, M Lindsay Lindsay Grayson, Paul Johnson, Benjamin Peter Howden, Timothy Paul Stinear

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87 Citations (Scopus)

Abstract

Vancomycin-resistant enterococci (VRE) are one of the leading causes of nosocomial infections in health care facilities around the globe. In particular, infections caused by vancomycin-resistant Enterococcus faecium are becoming increasingly common. Comparative and functional genomic studies of E. faecium isolates have so far been limited owing to the lack of a fully assembled E. faecium genome sequence. Here we address this issue and report the complete 3.0-Mb genome sequence of the multilocus sequence type 17 vancomycin-resistant Enterococcus faecium strain Aus0004, isolated from the bloodstream of a patient in Melbourne, Australia, in 1998. The genome comprises a 2.9-Mb circular chromosome and three circular plasmids. The chromosome harbors putative E. faecium virulence factors such as enterococcal surface protein, hemolysin, and collagen-binding adhesin. Aus0004 has a very large accessory genome (38 ) that includes three prophage and two genomic islands absent among 22 other E. faecium genomes. One of the prophage was present as inverted 50-kb repeats that appear to have facilitated a 683-kb chromosomal inversion across the replication terminus, resulting in a striking replichore imbalance. Other distinctive features include 76 insertion sequence elements and a single chromosomal copy of Tn1549 containing the vanB vancomycin resistance element. A complete E. faecium genome will be a useful resource to assist our understanding of this emerging nosocomial pathogen.
Original languageEnglish
Pages (from-to)2334 - 2341
Number of pages8
JournalJournal of Bacteriology
Volume194
Issue number9
DOIs
Publication statusPublished - 2012

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