Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens

Rukmali Wijayarathna, David M de Kretser, Rajini Sreenivasan, Helen Ludlow, Ralf Middendorff, Andreas Meinhardt, Kate L Loveland, Mark P Hedger

Research output: Research - peer-reviewArticle

Abstract

Activin A regulates testicular and epididymal development, but the role of activin B in the epididymis and vas deferens is unknown. Mouse models with reduced activin A (Inhba+/- and InhbaBK/+), or its complete absence (InhbaBK/BK), were investigated to identify specific roles of activins in the male reproductive tract. In 8 week old Inhba+/- mice, serum activin A decreased by 70%, with a 50% reduction of gene expression and protein in the testis, epididymis and vas deferens. Activin B and the activin-binding protein, follistatin, were similar to wild-type. Testis weights were slightly reduced in Inhba+/- mice, but the epididymis and vas deferens were normal, while the mice were fertile. Activin A was decreased by 70% in the serum, testis, epididymis and vas deferens of InhbaBK/+ mice and was undetectable in InhbaBK/BK mice, but activin B and follistatin levels were similar to wild-type. In 6 week old InhbaBK/BK mice, testis weights were 60% lower and epididymal weights were 50% lower than in either InhbaBK/+ or wild-type mice. The cauda epididymal epithelium showed infoldings and less intraluminal sperm, similar to 3.5 week old wild-type mice, but at 8 weeks no structural differences in the testis or epididymis were noted between InhbaBK/BK and wild-type mice. Thus, Inhbb can compensate for Inhba in regulating epididymal morphology, although testis and epididymal maturation is delayed in mice lacking Inhba Crucially, reduction or absence of activin A, at least in the presence of normal activin B levels, does not lead to major defects in the adult epididymis or vas deferens.
LanguageEnglish
JournalReproduction
DOIs
StateAccepted/In press - 13 Oct 2017

Cite this

@article{dd2164fd7aca43b2a02210ecd6b683fa,
title = "Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens",
abstract = "Activin A regulates testicular and epididymal development, but the role of activin B in the epididymis and vas deferens is unknown. Mouse models with reduced activin A (Inhba+/- and InhbaBK/+), or its complete absence (InhbaBK/BK), were investigated to identify specific roles of activins in the male reproductive tract. In 8 week old Inhba+/- mice, serum activin A decreased by 70%, with a 50% reduction of gene expression and protein in the testis, epididymis and vas deferens. Activin B and the activin-binding protein, follistatin, were similar to wild-type. Testis weights were slightly reduced in Inhba+/- mice, but the epididymis and vas deferens were normal, while the mice were fertile. Activin A was decreased by 70% in the serum, testis, epididymis and vas deferens of InhbaBK/+ mice and was undetectable in InhbaBK/BK mice, but activin B and follistatin levels were similar to wild-type. In 6 week old InhbaBK/BK mice, testis weights were 60% lower and epididymal weights were 50% lower than in either InhbaBK/+ or wild-type mice. The cauda epididymal epithelium showed infoldings and less intraluminal sperm, similar to 3.5 week old wild-type mice, but at 8 weeks no structural differences in the testis or epididymis were noted between InhbaBK/BK and wild-type mice. Thus, Inhbb can compensate for Inhba in regulating epididymal morphology, although testis and epididymal maturation is delayed in mice lacking Inhba Crucially, reduction or absence of activin A, at least in the presence of normal activin B levels, does not lead to major defects in the adult epididymis or vas deferens.",
author = "Rukmali Wijayarathna and {de Kretser}, {David M} and Rajini Sreenivasan and Helen Ludlow and Ralf Middendorff and Andreas Meinhardt and Loveland, {Kate L} and Hedger, {Mark P}",
year = "2017",
month = "10",
doi = "10.1530/REP-17-0485",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd",

}

Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens. / Wijayarathna, Rukmali; de Kretser, David M; Sreenivasan, Rajini; Ludlow, Helen; Middendorff, Ralf; Meinhardt, Andreas; Loveland, Kate L; Hedger, Mark P.

In: Reproduction, 13.10.2017.

Research output: Research - peer-reviewArticle

TY - JOUR

T1 - Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens

AU - Wijayarathna,Rukmali

AU - de Kretser,David M

AU - Sreenivasan,Rajini

AU - Ludlow,Helen

AU - Middendorff,Ralf

AU - Meinhardt,Andreas

AU - Loveland,Kate L

AU - Hedger,Mark P

PY - 2017/10/13

Y1 - 2017/10/13

N2 - Activin A regulates testicular and epididymal development, but the role of activin B in the epididymis and vas deferens is unknown. Mouse models with reduced activin A (Inhba+/- and InhbaBK/+), or its complete absence (InhbaBK/BK), were investigated to identify specific roles of activins in the male reproductive tract. In 8 week old Inhba+/- mice, serum activin A decreased by 70%, with a 50% reduction of gene expression and protein in the testis, epididymis and vas deferens. Activin B and the activin-binding protein, follistatin, were similar to wild-type. Testis weights were slightly reduced in Inhba+/- mice, but the epididymis and vas deferens were normal, while the mice were fertile. Activin A was decreased by 70% in the serum, testis, epididymis and vas deferens of InhbaBK/+ mice and was undetectable in InhbaBK/BK mice, but activin B and follistatin levels were similar to wild-type. In 6 week old InhbaBK/BK mice, testis weights were 60% lower and epididymal weights were 50% lower than in either InhbaBK/+ or wild-type mice. The cauda epididymal epithelium showed infoldings and less intraluminal sperm, similar to 3.5 week old wild-type mice, but at 8 weeks no structural differences in the testis or epididymis were noted between InhbaBK/BK and wild-type mice. Thus, Inhbb can compensate for Inhba in regulating epididymal morphology, although testis and epididymal maturation is delayed in mice lacking Inhba Crucially, reduction or absence of activin A, at least in the presence of normal activin B levels, does not lead to major defects in the adult epididymis or vas deferens.

AB - Activin A regulates testicular and epididymal development, but the role of activin B in the epididymis and vas deferens is unknown. Mouse models with reduced activin A (Inhba+/- and InhbaBK/+), or its complete absence (InhbaBK/BK), were investigated to identify specific roles of activins in the male reproductive tract. In 8 week old Inhba+/- mice, serum activin A decreased by 70%, with a 50% reduction of gene expression and protein in the testis, epididymis and vas deferens. Activin B and the activin-binding protein, follistatin, were similar to wild-type. Testis weights were slightly reduced in Inhba+/- mice, but the epididymis and vas deferens were normal, while the mice were fertile. Activin A was decreased by 70% in the serum, testis, epididymis and vas deferens of InhbaBK/+ mice and was undetectable in InhbaBK/BK mice, but activin B and follistatin levels were similar to wild-type. In 6 week old InhbaBK/BK mice, testis weights were 60% lower and epididymal weights were 50% lower than in either InhbaBK/+ or wild-type mice. The cauda epididymal epithelium showed infoldings and less intraluminal sperm, similar to 3.5 week old wild-type mice, but at 8 weeks no structural differences in the testis or epididymis were noted between InhbaBK/BK and wild-type mice. Thus, Inhbb can compensate for Inhba in regulating epididymal morphology, although testis and epididymal maturation is delayed in mice lacking Inhba Crucially, reduction or absence of activin A, at least in the presence of normal activin B levels, does not lead to major defects in the adult epididymis or vas deferens.

U2 - 10.1530/REP-17-0485

DO - 10.1530/REP-17-0485

M3 - Article

JO - Reproduction

T2 - Reproduction

JF - Reproduction

SN - 1470-1626

ER -

Wijayarathna R, de Kretser DM, Sreenivasan R, Ludlow H, Middendorff R, Meinhardt A et al. Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens. Reproduction. 2017 Oct 13. Available from, DOI: 10.1530/REP-17-0485