@inbook{87aeff21962f4141bdb2429075013eb1,
title = "Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery",
abstract = "Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described.",
keywords = "Biomarkers, Depletion, Multiple affinity removal system, Serum, Tryptic digestion",
author = "Penno, {Megan A S} and Matthias Ernst and Peter Hoffmann",
year = "2012",
doi = "10.1007/978-1-61779-573-2_15",
language = "English",
isbn = "9781617795725",
volume = "854",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "207--220",
booktitle = "Difference Gel Electrophoresis (DIGE): Methods and Protocols",
address = "United States of America",
}