Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery

Megan A S Penno, Matthias Ernst, Peter Hoffmann

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

3 Citations (Scopus)


Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described.

Original languageEnglish
Title of host publicationDifference Gel Electrophoresis (DIGE): Methods and Protocols
PublisherHumana Press
Number of pages14
ISBN (Print)9781617795725
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Biomarkers
  • Depletion
  • Multiple affinity removal system
  • Serum
  • Tryptic digestion

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