Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties

James M. Rae, Susan J. Ramus, Mark Waltham, Jane E. Armes, Ian G. Campbell, Robert Clarke, Robert J. Barndt, Michael D. Johnson, Erik W. Thompson

Research output: Contribution to journalArticleResearchpeer-review

61 Citations (Scopus)

Abstract

Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.

Original languageEnglish
Pages (from-to)543-552
Number of pages10
JournalClinical and Experimental Metastasis
Volume21
Issue number6
DOIs
Publication statusPublished - 1 Oct 2004
Externally publishedYes

Keywords

  • Breast cancer
  • Cell lines
  • Chromosomal number
  • MDA-MB-435
  • Melanoma
  • Microsatellite analysis
  • Tyrosinase

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