Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

Sarah Paule, Katie Meehan, Adam Rainczuk, Andrew N Stephens, Gui-Ying Nie

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)


Background: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.

Results: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.

Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.
Original languageEnglish
Article number50
Number of pages6
JournalProteome Science
Publication statusPublished - 1 Sep 2011

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