Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

Sarah Paule, Katie Meehan, Adam Rainczuk, Andrew N Stephens, Gui-Ying Nie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.

Results: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.

Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.
Original languageEnglish
Article number50
Number of pages6
JournalProteome Science
Volume9
DOIs
Publication statusPublished - 1 Sep 2011

Cite this

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title = "Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells",
abstract = "Background: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.Results: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.",
author = "Sarah Paule and Katie Meehan and Adam Rainczuk and Stephens, {Andrew N} and Gui-Ying Nie",
year = "2011",
month = "9",
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doi = "10.1186/1477-5956-9-50",
language = "English",
volume = "9",
journal = "Proteome Science",
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publisher = "Springer-Verlag London Ltd.",

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Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells. / Paule, Sarah; Meehan, Katie; Rainczuk, Adam; Stephens, Andrew N; Nie, Gui-Ying.

In: Proteome Science, Vol. 9, 50, 01.09.2011.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

AU - Paule, Sarah

AU - Meehan, Katie

AU - Rainczuk, Adam

AU - Stephens, Andrew N

AU - Nie, Gui-Ying

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Background: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.Results: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.

AB - Background: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.Results: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.Conclusions: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184050/

U2 - 10.1186/1477-5956-9-50

DO - 10.1186/1477-5956-9-50

M3 - Article

VL - 9

JO - Proteome Science

JF - Proteome Science

SN - 1477-5956

M1 - 50

ER -