Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction

Stefano C Meliga, William Farrugia, Paul Allen Ramsland, Robert J Falconer

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Cold-induced precipitation of a monoclonal IgM cryoglobulin isolated from a patient with Waldenstrom s macroglobulinemia was observed to have a negative activation enthalpy. The rate of the reaction increased, as the temperature decreased. Differential scanning calorimetry of the monoclonal IgM showed precipitation as an inverted peak during a downward temperature scan. The transition temperature was between 14 and 15 ?C and was possibly concentration dependent. At temperatures below the transition the precipitation was best described by second-order kinetics. The difference in change in enthalpy between precipitation and disassociation suggests that cold-induced precipitation had a fast precipitation stage followed by a slower consolidation reaction. Negligible curvature of the Eyring plot suggested the precipitation reaction was dominated by van der Waal forces and hydrogen bonding. Conversely, during an upward temperature scan, disassociation was observed as a positive enthalpy peak. This reaction had two stages, a reaction undoing consolidation followed by heat-induced disassociation that had first-order kinetics. ? 2012 American Chemical Society.
Original languageEnglish
Pages (from-to)490 - 494
Number of pages5
JournalJournal of Physical Chemistry B
Volume117
Issue number2
DOIs
Publication statusPublished - 2013

Cite this

Meliga, Stefano C ; Farrugia, William ; Ramsland, Paul Allen ; Falconer, Robert J. / Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 2. pp. 490 - 494.
@article{a1afb578029c4f58bb6722f26a324bbb,
title = "Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction",
abstract = "Cold-induced precipitation of a monoclonal IgM cryoglobulin isolated from a patient with Waldenstrom s macroglobulinemia was observed to have a negative activation enthalpy. The rate of the reaction increased, as the temperature decreased. Differential scanning calorimetry of the monoclonal IgM showed precipitation as an inverted peak during a downward temperature scan. The transition temperature was between 14 and 15 ?C and was possibly concentration dependent. At temperatures below the transition the precipitation was best described by second-order kinetics. The difference in change in enthalpy between precipitation and disassociation suggests that cold-induced precipitation had a fast precipitation stage followed by a slower consolidation reaction. Negligible curvature of the Eyring plot suggested the precipitation reaction was dominated by van der Waal forces and hydrogen bonding. Conversely, during an upward temperature scan, disassociation was observed as a positive enthalpy peak. This reaction had two stages, a reaction undoing consolidation followed by heat-induced disassociation that had first-order kinetics. ? 2012 American Chemical Society.",
author = "Meliga, {Stefano C} and William Farrugia and Ramsland, {Paul Allen} and Falconer, {Robert J}",
year = "2013",
doi = "10.1021/jp309109k",
language = "English",
volume = "117",
pages = "490 -- 494",
journal = "Journal of Physical Chemistry B",
issn = "1520-6106",
publisher = "American Chemical Society",
number = "2",

}

Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction. / Meliga, Stefano C; Farrugia, William; Ramsland, Paul Allen; Falconer, Robert J.

In: Journal of Physical Chemistry B, Vol. 117, No. 2, 2013, p. 490 - 494.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Cold-induced precipitation of a monoclonal IgM: a negative activation enthalpy reaction

AU - Meliga, Stefano C

AU - Farrugia, William

AU - Ramsland, Paul Allen

AU - Falconer, Robert J

PY - 2013

Y1 - 2013

N2 - Cold-induced precipitation of a monoclonal IgM cryoglobulin isolated from a patient with Waldenstrom s macroglobulinemia was observed to have a negative activation enthalpy. The rate of the reaction increased, as the temperature decreased. Differential scanning calorimetry of the monoclonal IgM showed precipitation as an inverted peak during a downward temperature scan. The transition temperature was between 14 and 15 ?C and was possibly concentration dependent. At temperatures below the transition the precipitation was best described by second-order kinetics. The difference in change in enthalpy between precipitation and disassociation suggests that cold-induced precipitation had a fast precipitation stage followed by a slower consolidation reaction. Negligible curvature of the Eyring plot suggested the precipitation reaction was dominated by van der Waal forces and hydrogen bonding. Conversely, during an upward temperature scan, disassociation was observed as a positive enthalpy peak. This reaction had two stages, a reaction undoing consolidation followed by heat-induced disassociation that had first-order kinetics. ? 2012 American Chemical Society.

AB - Cold-induced precipitation of a monoclonal IgM cryoglobulin isolated from a patient with Waldenstrom s macroglobulinemia was observed to have a negative activation enthalpy. The rate of the reaction increased, as the temperature decreased. Differential scanning calorimetry of the monoclonal IgM showed precipitation as an inverted peak during a downward temperature scan. The transition temperature was between 14 and 15 ?C and was possibly concentration dependent. At temperatures below the transition the precipitation was best described by second-order kinetics. The difference in change in enthalpy between precipitation and disassociation suggests that cold-induced precipitation had a fast precipitation stage followed by a slower consolidation reaction. Negligible curvature of the Eyring plot suggested the precipitation reaction was dominated by van der Waal forces and hydrogen bonding. Conversely, during an upward temperature scan, disassociation was observed as a positive enthalpy peak. This reaction had two stages, a reaction undoing consolidation followed by heat-induced disassociation that had first-order kinetics. ? 2012 American Chemical Society.

UR - http://www.ncbi.nlm.nih.gov/pubmed/23240622

U2 - 10.1021/jp309109k

DO - 10.1021/jp309109k

M3 - Article

VL - 117

SP - 490

EP - 494

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

SN - 1520-6106

IS - 2

ER -