Coherent interaction of optical second harmonic generation in collagen fibrils

Shi Wei Chu, Shih Peng Tai, Ming Che Chan, Chi Kuang Sun, I. Ching Hsiao, Chi Hung Lin, Yung Chih Chen, Bai Ling Lin

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

1 Citation (Scopus)

Abstract

We present the first experimental comparison between optical second harmonic generation images and atomic force microscope images in a matrix of nano-scaled collagen fibrils. Substantial variation of forward and backward propagated second harmonic generation radiation is observed in a single collagen fibril and is nicely correlated with the accurately determined thickness from an atomic force microscope. Contradicting to conventional nonlinear optical theory, our result indicates a linear relationship between fibril thickness and forward / backward second harmonic generation ratio. This is the first demonstration of estimating fibril thickness with nanometer precision by a noninvasive optical method.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences VIII
EditorsAmmasi Periasamy, Peter T. C. So
Number of pages9
DOIs
Publication statusPublished - 12 Mar 2008
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, United States of America
Duration: 20 Jan 200822 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6860
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences VIII
Country/TerritoryUnited States of America
CitySan Jose
Period20/01/0822/01/08

Keywords

  • Atomic force microscope
  • Nonlinear scattering
  • Optical interference
  • Radiation pattern
  • Three-dimensional microscopy
  • Type I collagen

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