Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting

Wenjie Zhang; Quinn A. Besford; Andrew J. Christofferson; Patrick Charchar; Joseph J. Richardson; Aaron Elbourne; Kristian Kempe; Christoph E. Hagemeyer; Matthew R. Field; Chris F. Mcconville; Irene Yarovsky; Frank Caruso

doi:10.1021/acs.nanolett.0c00295

Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting

Wenjie Zhang, Quinn A. Besford, Andrew J. Christofferson, Patrick Charchar, Joseph J. Richardson, Aaron Elbourne, Kristian Kempe, Christoph E. Hagemeyer, Matthew R. Field, Chris F. Mcconville, Irene Yarovsky, Frank Caruso

Research output: Contribution to journal › Article › Research › peer-review

53 Citations (Scopus)

Abstract

The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (Fe^III, Co^II, Cu^II, Ni^II, or Zn^II) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with Co^II systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to specifically interact with antibodies, which were investigated by molecular dynamics simulations. A model antibody fragment crystallizable (Fc) region in solution with Co^II-tannic acid complexes revealed that the solvent-exposed Co^II can directly coordinate to the histidine-rich portion of the Fc region. This one-pot interaction suggests anchoring of the antibody Fc region to the MPN on nanoparticles, allowing for enhanced targeting.

Original language	English
Pages (from-to)	2660-2666
Number of pages	7
Journal	Nano Letters
Volume	20
Issue number	4
DOIs	https://doi.org/10.1021/acs.nanolett.0c00295
Publication status	Published - 8 Apr 2020

Keywords

antibodies
metal-phenolic networks
molecular modeling
self-assembly
targeting

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10.1021/acs.nanolett.0c00295

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Zhang, W., Besford, Q. A., Christofferson, A. J., Charchar, P., Richardson, J. J., Elbourne, A., Kempe, K., Hagemeyer, C. E., Field, M. R., Mcconville, C. F., Yarovsky, I., & Caruso, F. (2020). Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting. Nano Letters, 20(4), 2660-2666. https://doi.org/10.1021/acs.nanolett.0c00295

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title = "Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting",

abstract = "The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with CoII systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to specifically interact with antibodies, which were investigated by molecular dynamics simulations. A model antibody fragment crystallizable (Fc) region in solution with CoII-tannic acid complexes revealed that the solvent-exposed CoII can directly coordinate to the histidine-rich portion of the Fc region. This one-pot interaction suggests anchoring of the antibody Fc region to the MPN on nanoparticles, allowing for enhanced targeting.",

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author = "Wenjie Zhang and Besford, {Quinn A.} and Christofferson, {Andrew J.} and Patrick Charchar and Richardson, {Joseph J.} and Aaron Elbourne and Kristian Kempe and Hagemeyer, {Christoph E.} and Field, {Matthew R.} and Mcconville, {Chris F.} and Irene Yarovsky and Frank Caruso",

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doi = "10.1021/acs.nanolett.0c00295",

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AU - Richardson, Joseph J.

AU - Elbourne, Aaron

AU - Kempe, Kristian

AU - Hagemeyer, Christoph E.

AU - Field, Matthew R.

AU - Mcconville, Chris F.

AU - Yarovsky, Irene

AU - Caruso, Frank

PY - 2020/4/8

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N2 - The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with CoII systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to specifically interact with antibodies, which were investigated by molecular dynamics simulations. A model antibody fragment crystallizable (Fc) region in solution with CoII-tannic acid complexes revealed that the solvent-exposed CoII can directly coordinate to the histidine-rich portion of the Fc region. This one-pot interaction suggests anchoring of the antibody Fc region to the MPN on nanoparticles, allowing for enhanced targeting.

AB - The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with CoII systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to specifically interact with antibodies, which were investigated by molecular dynamics simulations. A model antibody fragment crystallizable (Fc) region in solution with CoII-tannic acid complexes revealed that the solvent-exposed CoII can directly coordinate to the histidine-rich portion of the Fc region. This one-pot interaction suggests anchoring of the antibody Fc region to the MPN on nanoparticles, allowing for enhanced targeting.

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Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting

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Keywords

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Smart Targeted Nanoparticles for Diagnosis and Therapy

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

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Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting

Abstract

Keywords

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Smart Targeted Nanoparticles for Diagnosis and Therapy

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

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