Coagulation-induced shedding of platelet glycoprotein VI mediated by factor Xa

Mohammad Al-Tamimi, George Grigoriadis, Huy Tran, Eldho Paul, Patricia Servadei, Michael Berndt, Elizabeth Ellen Gardiner, Robert Keith Andrews

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study evaluated shedding of the platelet collagen receptor, glycoprotein VI (GPVI) in human plasma. Collagen or other ligands induce metalloproteinase-mediated GPVI ectodomain shedding, generating approximately 55-kDa soluble GPVI (sGPVI) and approximately 10-kDa platelet-associated fragments. In the absence of GPVI ligands, coagulation of platelet-rich plasma from healthy persons induced GPVI shedding, independent of added tissue factor, but inhibitable by metalloproteinase inhibitor, GM6001.
Original languageEnglish
Pages (from-to)3912 - 3920
Number of pages9
JournalBlood
Volume117
Issue number14
DOIs
Publication statusPublished - 2011

Cite this

Al-Tamimi, Mohammad ; Grigoriadis, George ; Tran, Huy ; Paul, Eldho ; Servadei, Patricia ; Berndt, Michael ; Gardiner, Elizabeth Ellen ; Andrews, Robert Keith. / Coagulation-induced shedding of platelet glycoprotein VI mediated by factor Xa. In: Blood. 2011 ; Vol. 117, No. 14. pp. 3912 - 3920.
@article{842060d066194e78b6bfd2203b310667,
title = "Coagulation-induced shedding of platelet glycoprotein VI mediated by factor Xa",
abstract = "This study evaluated shedding of the platelet collagen receptor, glycoprotein VI (GPVI) in human plasma. Collagen or other ligands induce metalloproteinase-mediated GPVI ectodomain shedding, generating approximately 55-kDa soluble GPVI (sGPVI) and approximately 10-kDa platelet-associated fragments. In the absence of GPVI ligands, coagulation of platelet-rich plasma from healthy persons induced GPVI shedding, independent of added tissue factor, but inhibitable by metalloproteinase inhibitor, GM6001.",
author = "Mohammad Al-Tamimi and George Grigoriadis and Huy Tran and Eldho Paul and Patricia Servadei and Michael Berndt and Gardiner, {Elizabeth Ellen} and Andrews, {Robert Keith}",
year = "2011",
doi = "10.1182/blood-2010-08-301523",
language = "English",
volume = "117",
pages = "3912 -- 3920",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "14",

}

Coagulation-induced shedding of platelet glycoprotein VI mediated by factor Xa. / Al-Tamimi, Mohammad; Grigoriadis, George; Tran, Huy; Paul, Eldho; Servadei, Patricia; Berndt, Michael; Gardiner, Elizabeth Ellen; Andrews, Robert Keith.

In: Blood, Vol. 117, No. 14, 2011, p. 3912 - 3920.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Coagulation-induced shedding of platelet glycoprotein VI mediated by factor Xa

AU - Al-Tamimi, Mohammad

AU - Grigoriadis, George

AU - Tran, Huy

AU - Paul, Eldho

AU - Servadei, Patricia

AU - Berndt, Michael

AU - Gardiner, Elizabeth Ellen

AU - Andrews, Robert Keith

PY - 2011

Y1 - 2011

N2 - This study evaluated shedding of the platelet collagen receptor, glycoprotein VI (GPVI) in human plasma. Collagen or other ligands induce metalloproteinase-mediated GPVI ectodomain shedding, generating approximately 55-kDa soluble GPVI (sGPVI) and approximately 10-kDa platelet-associated fragments. In the absence of GPVI ligands, coagulation of platelet-rich plasma from healthy persons induced GPVI shedding, independent of added tissue factor, but inhibitable by metalloproteinase inhibitor, GM6001.

AB - This study evaluated shedding of the platelet collagen receptor, glycoprotein VI (GPVI) in human plasma. Collagen or other ligands induce metalloproteinase-mediated GPVI ectodomain shedding, generating approximately 55-kDa soluble GPVI (sGPVI) and approximately 10-kDa platelet-associated fragments. In the absence of GPVI ligands, coagulation of platelet-rich plasma from healthy persons induced GPVI shedding, independent of added tissue factor, but inhibitable by metalloproteinase inhibitor, GM6001.

UR - http://bloodjournal.hematologylibrary.org/content/117/14/3912.full.pdf+html

U2 - 10.1182/blood-2010-08-301523

DO - 10.1182/blood-2010-08-301523

M3 - Article

VL - 117

SP - 3912

EP - 3920

JO - Blood

JF - Blood

SN - 0006-4971

IS - 14

ER -