Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1

Michelle M Hill, Noor H Daud, Cho S Aung, Dorothy Loo, Sally Martin, Samantha Murphy, Debra M Black, Rachael Barry, Fiona Simpson, Libin Liu, Paul F Pilch, John F Hancock, Marie-Odile Parat, Robert G Parton

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44 Citations (Scopus)

Abstract

Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCalpha caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.
Original languageEnglish
Article numbere43041
Number of pages9
JournalPLoS ONE
Volume7
Issue number8
DOIs
Publication statusPublished - 2012

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