Abstract
Immunoprecipitation is a technique which enables a macromolecule of interest to be isolated from heterogenous mixtures (particularly cell lysates). However, the immunoprecipitation of protein(s) can be challenging, with multiple variations of the basic technique required for successful antigenic pull-down. This depends on the target of interest, cell source, and localization. Here, immunoprecipitation of MIF from mouse and human macrophage cell lysates is described, which is both reliable and replicable, derived from multiple optimization experiments.
| Original language | English |
|---|---|
| Title of host publication | Macrophage Migration Inhibitory Factor |
| Subtitle of host publication | Methods and Protocols |
| Editors | James Harris, Eric Morand |
| Place of Publication | New York, NY |
| Publisher | Humana Press |
| Chapter | 10 |
| Pages | 115-122 |
| Number of pages | 8 |
| ISBN (Electronic) | 9781493999361 |
| ISBN (Print) | 9781493999354 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
Publication series
| Name | Methods in Molecular Biology: Springer Protocols |
|---|---|
| Publisher | Springer |
| Volume | 2080 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Keywords
- Co-immunoprecipitation
- Flowthrough
- Immunoprecipitation
- Macrophage migration inhibitory factor
- MIF
- Whole cell lysate
Cite this
Abidi, J. H., Harris, J., & Deen, N. S. (2020). Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 115-122). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_10