@inbook{8d1930cb6fb14f699a4f621344acf50f,
title = "Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor",
abstract = "Immunoprecipitation is a technique which enables a macromolecule of interest to be isolated from heterogenous mixtures (particularly cell lysates). However, the immunoprecipitation of protein(s) can be challenging, with multiple variations of the basic technique required for successful antigenic pull-down. This depends on the target of interest, cell source, and localization. Here, immunoprecipitation of MIF from mouse and human macrophage cell lysates is described, which is both reliable and replicable, derived from multiple optimization experiments.",
keywords = "Co-immunoprecipitation, Flowthrough, Immunoprecipitation, Macrophage migration inhibitory factor, MIF, Whole cell lysate",
author = "Abidi, {Jawad H.} and James Harris and Deen, {Nadia S.}",
year = "2020",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9936-1_10",
language = "English",
isbn = "9781493999354",
series = "Methods in Molecular Biology: Springer Protocols",
publisher = "Humana Press",
pages = "115--122",
editor = "Harris, {James } and Morand, {Eric }",
booktitle = "Macrophage Migration Inhibitory Factor",
address = "United States of America",
}