Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor

Jawad H. Abidi, James Harris, Nadia S. Deen

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Immunoprecipitation is a technique which enables a macromolecule of interest to be isolated from heterogenous mixtures (particularly cell lysates). However, the immunoprecipitation of protein(s) can be challenging, with multiple variations of the basic technique required for successful antigenic pull-down. This depends on the target of interest, cell source, and localization. Here, immunoprecipitation of MIF from mouse and human macrophage cell lysates is described, which is both reliable and replicable, derived from multiple optimization experiments.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric Morand
Place of PublicationNew York, NY
PublisherHumana Press
Chapter10
Pages115-122
Number of pages8
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Co-immunoprecipitation
  • Flowthrough
  • Immunoprecipitation
  • Macrophage migration inhibitory factor
  • MIF
  • Whole cell lysate

Cite this

Abidi, J. H., Harris, J., & Deen, N. S. (2020). Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 115-122). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_10