Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium

Kjiana Elkje Schwab, Caroline Eve Gargett

Research output: Contribution to journalArticleResearchpeer-review

353 Citations (Scopus)

Abstract

BACKGROUND: Human endometrium has immense regenerative capacity, growing 5 mm in 7 days every month. We have previously identified a small population of colony-forming endometrial stromal cells which we hypothesize are mesenchymal stem cells (MSC). The aim of this study was to determine if the co-expression of two perivascular cell markers, CD146 and platelet-derived growth factor-receptor beta (PDGF-Rbeta), will prospectively isolate endometrial stromal cells which exhibit MSC properties, and determine their location in human endometrium. METHODS: Single cell suspensions of human endometrial stromal cells were fluorescence activated cell sorting (FACS) sorted into CD146(+)PDGF-Rbeta(+) and CD146(-)PDGF-Rbeta(-) populations and analysed for colony-forming ability, in vitro differentiation and expression of typical MSC markers. Full thickness human endometrial sections were co-stained for CD146 and PDGF-Rbeta. RESULTS: FACS stromal CD146(+)PDGF-Rbeta(+) stromal cells (1.5 of sorted population) were enriched for colony-forming cells compared with CD146(-)PDGF-Rbeta(-) cells (7.7 +/- 1.7 versus 0.7 +/- 0.2 P
Original languageEnglish
Pages (from-to)2903 - 2911
Number of pages9
JournalHuman Reproduction
Volume22
Issue number11
Publication statusPublished - 2007

Cite this