Abstract
Problem addressed: Clostridium perfringens is the etiological agent of necrotic enteritis in chickens. As necrotic enteritis is a gastrointestinal disease, the interactions of pathogenic C. perfringens strains with the complex microbiota of the gastrointestinal tract may influence disease development and severity of disease. Objective: In this study the interactions of a pathogenic strain of C. perfringens, WER-NE36, with the microbiota of broilers was investigated to determine whether the pre-existing microbiota could influence disease outcomes in the necrotic enteritis challenge model. Methods and approach: Faecal microbiota compositions were measured before and after C. perfringens challenge and caecal microbiota was also characterised at necropsy. The microbiota profiles from individual birds were related back to the degree of necrotic enteritis that each bird developed. Results: Under the experimental conditions used the pre-existing microbiota did not have an effect on disease outcomes. However, C. perfringens challenge was shown to have a significant effect on the microbiota of broilers, regardless of disease status, by displacement of commensal clostridia. Conclusions: The microbiota signature after challenge resembled that of lower productivity birds, supporting the finding that physically obvious disease (necrotic lesions), as well as dysbiosis, are associated with shifts in gut microbiota and affect broiler performance, increasing costs to the poultry industry.
| Original language | English |
|---|---|
| Pages (from-to) | 119-126 |
| Number of pages | 8 |
| Journal | Veterinary Microbiology |
| Volume | 227 |
| DOIs | |
| Publication status | Published - 1 Dec 2018 |
Keywords
- 16S rRNA
- Chicken
- Clostridium perfringens
- Dysbiosis
- Microbiota
- Necrotic enteritis
Cite this
}
Clostridium perfringens-mediated necrotic enteritis is not influenced by the pre-existing microbiota but is promoted by large changes in the post-challenge microbiota. / Lacey, Jake Aidan; Stanley, Dragana; Keyburn, Anthony L; Ford, Mark; Chen, Honglei; Johanesen, Priscilla ; Lyras, Dena; Moore, Robert J.
In: Veterinary Microbiology, Vol. 227, 01.12.2018, p. 119-126.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Clostridium perfringens-mediated necrotic enteritis is not influenced by the pre-existing microbiota but is promoted by large changes in the post-challenge microbiota
AU - Lacey, Jake Aidan
AU - Stanley, Dragana
AU - Keyburn, Anthony L
AU - Ford, Mark
AU - Chen, Honglei
AU - Johanesen, Priscilla
AU - Lyras, Dena
AU - Moore, Robert J.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Problem addressed: Clostridium perfringens is the etiological agent of necrotic enteritis in chickens. As necrotic enteritis is a gastrointestinal disease, the interactions of pathogenic C. perfringens strains with the complex microbiota of the gastrointestinal tract may influence disease development and severity of disease. Objective: In this study the interactions of a pathogenic strain of C. perfringens, WER-NE36, with the microbiota of broilers was investigated to determine whether the pre-existing microbiota could influence disease outcomes in the necrotic enteritis challenge model. Methods and approach: Faecal microbiota compositions were measured before and after C. perfringens challenge and caecal microbiota was also characterised at necropsy. The microbiota profiles from individual birds were related back to the degree of necrotic enteritis that each bird developed. Results: Under the experimental conditions used the pre-existing microbiota did not have an effect on disease outcomes. However, C. perfringens challenge was shown to have a significant effect on the microbiota of broilers, regardless of disease status, by displacement of commensal clostridia. Conclusions: The microbiota signature after challenge resembled that of lower productivity birds, supporting the finding that physically obvious disease (necrotic lesions), as well as dysbiosis, are associated with shifts in gut microbiota and affect broiler performance, increasing costs to the poultry industry.
AB - Problem addressed: Clostridium perfringens is the etiological agent of necrotic enteritis in chickens. As necrotic enteritis is a gastrointestinal disease, the interactions of pathogenic C. perfringens strains with the complex microbiota of the gastrointestinal tract may influence disease development and severity of disease. Objective: In this study the interactions of a pathogenic strain of C. perfringens, WER-NE36, with the microbiota of broilers was investigated to determine whether the pre-existing microbiota could influence disease outcomes in the necrotic enteritis challenge model. Methods and approach: Faecal microbiota compositions were measured before and after C. perfringens challenge and caecal microbiota was also characterised at necropsy. The microbiota profiles from individual birds were related back to the degree of necrotic enteritis that each bird developed. Results: Under the experimental conditions used the pre-existing microbiota did not have an effect on disease outcomes. However, C. perfringens challenge was shown to have a significant effect on the microbiota of broilers, regardless of disease status, by displacement of commensal clostridia. Conclusions: The microbiota signature after challenge resembled that of lower productivity birds, supporting the finding that physically obvious disease (necrotic lesions), as well as dysbiosis, are associated with shifts in gut microbiota and affect broiler performance, increasing costs to the poultry industry.
KW - 16S rRNA
KW - Chicken
KW - Clostridium perfringens
KW - Dysbiosis
KW - Microbiota
KW - Necrotic enteritis
UR - http://www.scopus.com/inward/record.url?scp=85056492103&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2018.10.022
DO - 10.1016/j.vetmic.2018.10.022
M3 - Article
VL - 227
SP - 119
EP - 126
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -